Although next-generation sequencing has revolutionized the ability to associate variants with
human diseases, diagnostic rates and development of new therapies are still limited by a …

The function of the majority of genes in the mouse and human genomes remains unknown.
The mouse embryonic stem cell knockout resource provides a basis for the characterization …

The CRISPR system is widely used in genome editing for biomedical research. Here, using
either dual paired Cas9 D10A nickases or paired Cas9 nuclease we characterize …

The application of CRISPR/Cas9 technology has revolutionised genetics by greatly enhancing
the efficacy of genome editing in the early embryo. Furthermore, the system has enabled …

Background Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9) genome editing have led to the use of long …

The International Mouse Phenotyping Consortium reports the generation of new mouse
mutant strains for more than 5,000 genes, including 2,850 novel null, 2,987 novel …

The suprachiasmatic nucleus (SCN) coordinates circadian rhythms that adapt the individual
to solar time. SCN pacemaking revolves around feedback loops in which expression of …

Background Recent developments in CRISPR/Cas9 genome-editing tools have facilitated
the introduction of precise alleles, including genetic intervals spanning several kilobases, …

Hearing relies on mechanically gated ion channels present in the actin‐rich stereocilia bundles
at the apical surface of cochlear hair cells. Our knowledge of the mechanisms underlying …

Background Karyotypic integrity is essential for the successful germline transmission of
alleles mutated in embryonic stem (ES) cells. Classical methods for the identification of …