The assembly of DNA from small fragments into large constructs has seen significant recent
development, becoming a pivotal technology in the ability to implement the vision of …

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but
cannot be compared between instruments without a standardized calibration protocol and is …

Overlap-directed DNA assembly methods allow multiple DNA parts to be assembled together
in one reaction. These methods, which rely on sequence homology between the ends of …

The ability to quickly and reliably assemble DNA constructs is one of the key enabling
technologies for synthetic biology. Here we define a new Biopart Assembly Standard for …

DNA assembly is a key part of constructing gene expression systems and even whole
chromosomes. In the past decade, a plethora of powerful new DNA assembly methods — …

Synthetic biology research and its industrial applications rely on deterministic spatiotemporal
control of gene expression. Recently, electrochemical control of gene expression has been …

Natural transformation is a dominant force in bacterial evolution by promoting horizontal
gene transfer. This process may have devastating consequences, such as the spread of …

Multi-part DNA assembly is the physical starting point for many projects in Synthetic and
Molecular Biology. The ability to explore a genetic design space by building extensive libraries …

The structure and biological function of the DNA double helix are based on interactions
recognizing sequence complementarity between two single strands of DNA. A single DNA strand …

R2oDNA Designer is a web application that stochastically generates orthogonal sets of
synthetic DNA sequences designed to be biologically neutral. Biologically neutral sequences …