[HTML][HTML] Double-membrane vesicles as platforms for viral replication
G Wolff, CE Melia, EJ Snijder, M Bárcena - Trends in microbiology, 2020 - cell.com
Viruses, as obligate intracellular parasites, exploit cellular pathways and resources in a
variety of fascinating ways. A striking example of this is the remodelling of intracellular …
variety of fascinating ways. A striking example of this is the remodelling of intracellular …
A molecular pore spans the double membrane of the coronavirus replication organelle
G Wolff, RWAL Limpens, JC Zevenhoven-Dobbe… - Science, 2020 - science.org
Coronavirus genome replication is associated with virus-induced cytosolic double-membrane
vesicles, which may provide a tailored microenvironment for viral RNA synthesis in the …
vesicles, which may provide a tailored microenvironment for viral RNA synthesis in the …
[HTML][HTML] Mind the gap: Micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae
G Wolff, RWAL Limpens, S Zheng, EJ Snijder… - Journal of structural …, 2019 - Elsevier
Cryo-focused ion beam (FIB)-milling of biological samples can be used to generate thin
electron-transparent slices from cells grown or deposited on EM grids. These so called cryo-…
electron-transparent slices from cells grown or deposited on EM grids. These so called cryo-…
[HTML][HTML] AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction
AreTomo, an abbreviation for Alignment and Reconstruction for Electron Tomography, is a
GPU accelerated software package that fully automates motion-corrected marker-free …
GPU accelerated software package that fully automates motion-corrected marker-free …
[HTML][HTML] Virtual and augmented reality in critical care medicine: the patient's, clinician's, and researcher's perspective
Virtual reality (VR) and augmented reality (AR) are aspiring, new technologies with
increasing use in critical care medicine. While VR fully immerses the user into a virtual three-…
increasing use in critical care medicine. While VR fully immerses the user into a virtual three-…
Survival benefits of invasive versus conservative strategies in heart failure in patients with reduced ejection fraction and coronary artery disease: a meta-analysis
G Wolff, D Dimitroulis, F Andreotti… - Circulation: Heart …, 2017 - Am Heart Assoc
Background— Heart failure with reduced ejection fraction caused by ischemic heart disease
is associated with increased morbidity and mortality. It remains unclear whether …
is associated with increased morbidity and mortality. It remains unclear whether …
[HTML][HTML] The impact of frailty on survival in elderly intensive care patients with COVID-19: the COVIP study
…, A Artigas, B Bollen Pinto, JC Schefold, G Wolff… - Critical care, 2021 - Springer
Background The COVID-19 pandemic has led highly developed healthcare systems to the
brink of collapse due to the large numbers of patients being admitted into hospitals. One of …
brink of collapse due to the large numbers of patients being admitted into hospitals. One of …
Towards correlative super‐resolution fluorescence and electron cryo‐microscopy
G Wolff, C Hagen, K Grünewald… - Biology of the Cell, 2016 - Wiley Online Library
Correlative light and electron microscopy (CLEM) has become a powerful tool in life
sciences. Particularly cryo‐CLEM, the combination of fluorescence cryo‐microscopy (cryo‐FM) …
sciences. Particularly cryo‐CLEM, the combination of fluorescence cryo‐microscopy (cryo‐FM) …
Virtual and augmented reality in cardiovascular care: state-of-the-art and future perspectives
Applications of virtual reality (VR) and augmented reality (AR) assist both health care providers
and patients in cardiovascular education, complementing traditional learning methods. …
and patients in cardiovascular education, complementing traditional learning methods. …
Steroid use in elderly critically ill COVID-19 patients
…, BB Pinto, JC Schefold, G Wolff… - European respiratory …, 2021 - Eur Respiratory Soc
More than a year after the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
pandemic, treating patients with coronavirus disease 2019 (COVID-19) remains …
pandemic, treating patients with coronavirus disease 2019 (COVID-19) remains …