[HTML][HTML] Double-membrane vesicles as platforms for viral replication

G Wolff, CE Melia, EJ Snijder, M Bárcena - Trends in microbiology, 2020 - cell.com
Viruses, as obligate intracellular parasites, exploit cellular pathways and resources in a
variety of fascinating ways. A striking example of this is the remodelling of intracellular …

A molecular pore spans the double membrane of the coronavirus replication organelle

G Wolff, RWAL Limpens, JC Zevenhoven-Dobbe… - Science, 2020 - science.org
Coronavirus genome replication is associated with virus-induced cytosolic double-membrane
vesicles, which may provide a tailored microenvironment for viral RNA synthesis in the …

[HTML][HTML] Mind the gap: Micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae

G Wolff, RWAL Limpens, S Zheng, EJ Snijder… - Journal of structural …, 2019 - Elsevier
Cryo-focused ion beam (FIB)-milling of biological samples can be used to generate thin
electron-transparent slices from cells grown or deposited on EM grids. These so called cryo-…

[HTML][HTML] AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction

S Zheng, G Wolff, G Greenan, Z Chen, FGA Faas… - Journal of Structural …, 2022 - Elsevier
AreTomo, an abbreviation for Alignment and Reconstruction for Electron Tomography, is a
GPU accelerated software package that fully automates motion-corrected marker-free …

[HTML][HTML] Virtual and augmented reality in critical care medicine: the patient's, clinician's, and researcher's perspective

RR Bruno, G Wolff, B Wernly, M Masyuk, K Piayda… - Critical Care, 2022 - Springer
Virtual reality (VR) and augmented reality (AR) are aspiring, new technologies with
increasing use in critical care medicine. While VR fully immerses the user into a virtual three-…

Survival benefits of invasive versus conservative strategies in heart failure in patients with reduced ejection fraction and coronary artery disease: a meta-analysis

G Wolff, D Dimitroulis, F Andreotti… - Circulation: Heart …, 2017 - Am Heart Assoc
Background— Heart failure with reduced ejection fraction caused by ischemic heart disease
is associated with increased morbidity and mortality. It remains unclear whether …

[HTML][HTML] The impact of frailty on survival in elderly intensive care patients with COVID-19: the COVIP study

…, A Artigas, B Bollen Pinto, JC Schefold, G Wolff… - Critical care, 2021 - Springer
Background The COVID-19 pandemic has led highly developed healthcare systems to the
brink of collapse due to the large numbers of patients being admitted into hospitals. One of …

Towards correlative super‐resolution fluorescence and electron cryo‐microscopy

G Wolff, C Hagen, K Grünewald… - Biology of the Cell, 2016 - Wiley Online Library
Correlative light and electron microscopy (CLEM) has become a powerful tool in life
sciences. Particularly cryo‐CLEM, the combination of fluorescence cryo‐microscopy (cryo‐FM) …

Virtual and augmented reality in cardiovascular care: state-of-the-art and future perspectives

C Jung, G Wolff, B Wernly, RR Bruno, M Franz… - Cardiovascular …, 2022 - jacc.org
Applications of virtual reality (VR) and augmented reality (AR) assist both health care providers
and patients in cardiovascular education, complementing traditional learning methods. …

Steroid use in elderly critically ill COVID-19 patients

…, BB Pinto, JC Schefold, G Wolff… - European respiratory …, 2021 - Eur Respiratory Soc
More than a year after the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
pandemic, treating patients with coronavirus disease 2019 (COVID-19) remains …