High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire
Each B-cell receptor consists of a pair of heavy and light chains. High-throughput sequencing
can identify large numbers of heavy- and light-chain variable regions (V H and V L ) in a …
can identify large numbers of heavy- and light-chain variable regions (V H and V L ) in a …
Virus-based toolkit for the directed synthesis of magnetic and semiconducting nanowires
…, ST Kottmann, RY Sweeney, A Hayhurst, G Georgiou… - Science, 2004 - science.org
We report a virus-based scaffold for the synthesis of single-crystal ZnS, CdS, and freestanding
chemically ordered CoPt and FePt nanowires, with the means of modifying substrate …
chemically ordered CoPt and FePt nanowires, with the means of modifying substrate …
[HTML][HTML] The promise and challenge of high-throughput sequencing of the antibody repertoire
G Georgiou, GC Ippolito, J Beausang, CE Busse… - Nature …, 2014 - nature.com
Efforts to determine the antibody repertoire encoded by B cells in the blood or lymphoid
organs using high-throughput DNA sequencing technologies have been advancing at an …
organs using high-throughput DNA sequencing technologies have been advancing at an …
CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy
Cancer immunotherapy restores or enhances the effector function of CD8 + T cells in the
tumour microenvironment 1 , 2 . CD8 + T cells activated by cancer immunotherapy clear …
tumour microenvironment 1 , 2 . CD8 + T cells activated by cancer immunotherapy clear …
Isolation of engineered, full-length antibodies from libraries expressed in Escherichia coli
…, T Van Blarcom, R Mabry, BL Iverson, G Georgiou - Nature …, 2007 - nature.com
We describe facile isolation of full-length IgG antibodies from combinatorial libraries expressed
in E. coli. Full-length heavy and light chains are secreted into the periplasm, where they …
in E. coli. Full-length heavy and light chains are secreted into the periplasm, where they …
The substance of accounting for public‐private partnerships
D Heald, G Georgiou - Financial Accountability & Management, 2011 - Wiley Online Library
This article evaluates Public‐Private Partnerships (PPP) accounting practice and the related
financial accounting and reporting requirements. Governments across the world are …
financial accounting and reporting requirements. Governments across the world are …
Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm
…, F Åslund, J Beckwith, G Georgiou - Proceedings of the …, 1999 - National Acad Sciences
Under physiological conditions, the Escherichia coli cytoplasm is maintained in a reduced
state that strongly disfavors the formation of stable disulfide bonds in proteins. However, …
state that strongly disfavors the formation of stable disulfide bonds in proteins. However, …
Cysteine depletion induces pancreatic tumor ferroptosis in mice
Ferroptosis is a form of cell death that results from the catastrophic accumulation of lipid
reactive oxygen species (ROS). Oncogenic signaling elevates lipid ROS production in many …
reactive oxygen species (ROS). Oncogenic signaling elevates lipid ROS production in many …
[HTML][HTML] Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies
…, IA Wilson, JP Moore, AB Ward, G Georgiou… - Nature, 2014 - nature.com
Antibodies capable of neutralizing HIV-1 often target variable regions 1 and 2 (V1V2) of the
HIV-1 envelope, but the mechanism of their elicitation has been unclear. Here we define the …
HIV-1 envelope, but the mechanism of their elicitation has been unclear. Here we define the …
Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines
G Georgiou, C Stathopoulos, PS Daugherty… - Nature …, 1997 - nature.com
In recent years there has been considerable progress towards the development of
expression systems for the display of heterologous polypeptides and, to a lesser extent, …
expression systems for the display of heterologous polypeptides and, to a lesser extent, …