The genome sequence of the solvent-producing bacteriumClostridium acetobutylicum
ATCC 824 has been determined by the shotgun approach. The genome consists of a 3.94-Mb …

Enterohemorrhagic strains of Escherichia coli must pass through the acidic gastric barrier to
cause gastrointestinal disease. Taking into account the apparent low infectious dose of …

Metabolic engineering studies have generally focused on manipulating enzyme levels
through either the amplification, addition, or deletion of a particular pathway. However, with …

We have previously cloned the acetone-formation pathway gene, encoding acetoacetate
decarboxylase (adc), and butyrate-formation pathway gene, encoding phosphotransbutyrylase (…

A novel in vivo method of producing succinate has been developed. A genetically engineered
Escherichia coli strain has been constructed to meet the NADH requirement and carbon …

Applications of genetic engineering or metabolic engineering have increased in both academic
and industrial institutions. Most current metabolic engineering studies have focused on …

The potential to produce succinate aerobically in Escherichia coli would offer great advantages
over anaerobic fermentation in terms of faster biomass generation, carbon throughput, …

Real-time chemical sensing is crucial for applications in environmental and health monitoring
1 . Biosensors can detect a variety of molecules through genetic circuits that use these …

Integrational plasmid technology has been used to disrupt metabolic pathways leading to
acetate and butyrate formation in Clostridium acetobutylicum ATCC 824. Non-replicative …

Clostridium acetobutylicum naturally produces acetone as well as butanol and ethanol. Since
acetone cannot be used as a biofuel, its production needs to be minimized or suppressed …