Purification of mammalian telomeric DNA for single-molecule analysis

G Mazzucco, A Huda, M Galli, E Zanella, Y Doksani - Nature Protocols, 2022 - nature.com
Here we provide a detailed protocol for the enrichment of telomeric repeats from mouse and
human cells. The procedure consists of two successive rounds of digestion with frequently …

[HTML][HTML] Telomere damage induces internal loops that generate telomeric circles

G Mazzucco, A Huda, M Galli, D Piccini… - Nature …, 2020 - nature.com
Extrachromosomal telomeric circles are commonly invoked as important players in telomere
maintenance, but their origin has remained elusive. Using electron microscopy analysis on …

The telomerase reverse transcriptase elongates reversed replication forks at telomeric repeats

A Huda, H Arakawa, G Mazzucco, M Galli… - Science …, 2023 - science.org
The telomerase reverse transcriptase elongates telomeres to prevent replicative senescence.
This process requires exposure of the 3′-end, which is thought to occur when two sister …

[HTML][HTML] Enrichment of centromeric DNA from human cells

R Gamba, G Mazzucco, T Wilhelm, L Velikovsky… - PLoS …, 2022 - journals.plos.org
Centromeres are key elements for chromosome segregation. Canonical centromeres are
built over long-stretches of tandem repetitive arrays. Despite being quite abundant compared …

[HTML][HTML] Application of Social-Life Cycle Assessment in Urban Settings: Social Impact Assessment of Green Roofs

G Mazzucco, M Canepa, K Perini - Buildings, 2023 - mdpi.com
Sustainability brings together three dimensions: environment, economy and society, as
according to the Agenda 21, a comprehensive plan of action to be taken globally, nationally, and …

A method to enrich and purify centromeric DNA from human cells

R Gamba, G Mazzucco, T Wilhelm, F Chardon… - bioRxiv, 2021 - biorxiv.org
Centromeres are key elements for chromosome segregation. Canonical centromeres are
built over long-stretches of tandem repetitive arrays. Despite being quite abundant compared …

[HTML][HTML] Chronic replication problems impact cell morphology and adhesion of DNA ligase I defective cells

…, M Oliverio, V Leva, S Bione, R Carriero, G Mazzucco… - PLoS …, 2015 - journals.plos.org
Moderate DNA damage resulting from metabolic activities or sub-lethal doses of exogenous
insults may eventually lead to cancer onset. Human 46BR.1G1 cells bear a mutation in …

Applicazione del Social-Life Cycle Assessment in ambito urbano: Valutazione dell'impatto sociale nelle coperture verdi

G Mazzucco - 2023 - unire.unige.it
La tesi propone il tema dell’applicazione di uno strumento – il Social-Life Cycle Assessment
– in ambito urbano. Lo studio promuove una metodologia semplificata per la valutazione …

Potere di controllo e tutela dei dati

G MAZZUCCO - thesis.unipd.it
Potere di controllo e tutela dei dati UNItesi UNItesi Home Sfoglia Macrotipologie & tipologie
Titolo Autore Relatore Anno Dipartimento Corso Correlatore IT Italiano Italiano English …

Enrichment of DNA replication intermediates by EdU pull down.

F Pessina, A Romussi, D Piccini, G Mazzucco… - Methods in Cell …, 2022 - europepmc.org
Analysis of replication fork structures in electron microscopy (EM) can provide important
mechanistic insights in DNA replication studies. A major challenge in this type of analysis is the …