The genetic interrogation and reprogramming of cells requires methods for robust and
precise targeting of genes for expression or repression. The CRISPR-associated catalytically …

Recent studies highlight the importance of translational control in determining protein
abundance, underscoring the value of measuring gene expression at the level of translation. We …

Ribosome profiling suggests that ribosomes occupy many regions of the transcriptome
thought to be noncoding, including 5′ UTRs and long noncoding RNAs (lncRNAs). Apparent …

Meiosis is a complex developmental process that generates haploid cells from diploid
progenitors. We measured messenger RNA (mRNA) abundance and protein production through …

Novel protein-coding genes can arise either through re-organization of pre-existing genes
or de novo 1 , 2 . Processes involving re-organization of pre-existing genes, notably after …

Ribosome profiling, which involves the deep sequencing of ribosome-protected mRNA
fragments, is a powerful tool for globally monitoring translation in vivo. The method has facilitated …

To better understand the gene regulatory mechanisms that program developmental processes,
we carried out simultaneous genome-wide measurements of mRNA, translation, and …

The conserved and essential DEAD-box RNA helicase Ded1p from yeast and its mammalian
orthologue DDX3 are critical for the initiation of translation 1 . Mutations in DDX3 are linked …

During meiosis, cohesins—protein complexes that hold sister chromatids together—are lost
from chromosomes in a step-wise manner 1 . Loss of cohesins from chromosome arms is …

The meiotic recombination checkpoint delays gamete precursors in G2 until DNA breaks
created during recombination are repaired and chromosome structure has been restored. Here, …