User profiles for Gloria A. Brar
Gloria BrarAssociate Professor, Molecular and Cell Biology, UC-Berkeley Verified email at berkeley.edu Cited by 9532 |
[HTML][HTML] CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes
The genetic interrogation and reprogramming of cells requires methods for robust and
precise targeting of genes for expression or repression. The CRISPR-associated catalytically …
precise targeting of genes for expression or repression. The CRISPR-associated catalytically …
The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments
Recent studies highlight the importance of translational control in determining protein
abundance, underscoring the value of measuring gene expression at the level of translation. We …
abundance, underscoring the value of measuring gene expression at the level of translation. We …
[HTML][HTML] Ribosome profiling reveals pervasive translation outside of annotated protein-coding genes
Ribosome profiling suggests that ribosomes occupy many regions of the transcriptome
thought to be noncoding, including 5′ UTRs and long noncoding RNAs (lncRNAs). Apparent …
thought to be noncoding, including 5′ UTRs and long noncoding RNAs (lncRNAs). Apparent …
High-resolution view of the yeast meiotic program revealed by ribosome profiling
Meiosis is a complex developmental process that generates haploid cells from diploid
progenitors. We measured messenger RNA (mRNA) abundance and protein production through …
progenitors. We measured messenger RNA (mRNA) abundance and protein production through …
Proto-genes and de novo gene birth
Novel protein-coding genes can arise either through re-organization of pre-existing genes
or de novo 1 , 2 . Processes involving re-organization of pre-existing genes, notably after …
or de novo 1 , 2 . Processes involving re-organization of pre-existing genes, notably after …
Ribosome profiling reveals the what, when, where and how of protein synthesis
GA Brar, JS Weissman - Nature reviews Molecular cell biology, 2015 - nature.com
Ribosome profiling, which involves the deep sequencing of ribosome-protected mRNA
fragments, is a powerful tool for globally monitoring translation in vivo. The method has facilitated …
fragments, is a powerful tool for globally monitoring translation in vivo. The method has facilitated …
[PDF][PDF] Pervasive, coordinated protein-level changes driven by transcript isoform switching during meiosis
To better understand the gene regulatory mechanisms that program developmental processes,
we carried out simultaneous genome-wide measurements of mRNA, translation, and …
we carried out simultaneous genome-wide measurements of mRNA, translation, and …
The helicase Ded1p controls use of near-cognate translation initiation codons in 5′ UTRs
The conserved and essential DEAD-box RNA helicase Ded1p from yeast and its mammalian
orthologue DDX3 are critical for the initiation of translation 1 . Mutations in DDX3 are linked …
orthologue DDX3 are critical for the initiation of translation 1 . Mutations in DDX3 are linked …
Rec8 phosphorylation and recombination promote the step-wise loss of cohesins in meiosis
During meiosis, cohesins—protein complexes that hold sister chromatids together—are lost
from chromosomes in a step-wise manner 1 . Loss of cohesins from chromosome arms is …
from chromosomes in a step-wise manner 1 . Loss of cohesins from chromosome arms is …
[HTML][HTML] The FK506 binding protein Fpr3 counteracts protein phosphatase 1 to maintain meiotic recombination checkpoint activity
The meiotic recombination checkpoint delays gamete precursors in G2 until DNA breaks
created during recombination are repaired and chromosome structure has been restored. Here, …
created during recombination are repaired and chromosome structure has been restored. Here, …