Characterizing the transcriptome of individual cells is fundamental to understanding complex
biological systems. We describe a droplet-based system that enables 3′ mRNA counting …

Several of the thousands of human long non-coding RNAs (lncRNAs) have been
functionally characterized 1 , 2 , 3 , 4 ; however, potential roles for lncRNAs in somatic tissue …

In vitro cancer cultures, including three-dimensional organoids, typically contain exclusively
neoplastic epithelium but require artificial reconstitution to recapitulate the tumor …

Haplotyping of human chromosomes is a prerequisite for cataloguing the full repertoire of
genetic variation. We present a microfluidics-based, linked-read sequencing technology that …

MicroRNAs (miRNAs) are short, highly conserved noncoding RNA molecules that repress
gene expression in a sequence-dependent manner. We performed single-cell measurements …

Understanding complex tissues requires single-cell deconstruction of gene regulation with
precision and scale. Here, we assess the performance of a massively parallel droplet-based …

The Genome in a Bottle Consortium, hosted by the National Institute of Standards and
Technology (NIST) is creating reference materials and data for human genome sequencing, as …

Short RNA expression in several distinct stages of T-lymphocyte development was
comprehensively profiled. The total number of microRNAs (miRNAs) expressed per cell at different …

Long noncoding RNAs (lncRNAs) regulate diverse processes, yet a potential role for
lncRNAs in maintaining the undifferentiated state in somatic tissue progenitor cells remains …

The distal lung contains terminal bronchioles and alveoli that facilitate gas exchange. Three-dimensional
in vitro human distal lung culture systems would strongly facilitate the …