One-third of all protein-coding genes from bacterial genomes cannot be annotated with a
function. Here, to investigate the functions of these genes, we present genome-wide mutant …

The pathway of electrons required for the reduction of sulfate in sulfate-reducing bacteria (SRB)
is not yet fully characterized. In order to determine the role of a transmembrane protein …

For many bacteria with sequenced genomes, we do not understand how they synthesize
some amino acids. This makes it challenging to reconstruct their metabolism, and has led to …

Human gene therapy has advanced from twentieth-century conception to twenty-first-century
reality. The recombinant Adeno-Associated Virus (rAAV) is a major gene therapy vector. …

Many species have evolved to function as specialized mutualists, often to the detriment of
their ability to survive independently. However, there are few, if any, well-controlled …

Crp/Fnr-type global transcriptional regulators regulate various metabolic pathways in bacteria
and typically function in response to environmental changes. However, little is known …

… in buffer solution containing 180 μL 5 M NaCl, 20 μL 1 M Tris HCl, 449 μL double deionized
(… Grant M. Zane designed and performed experiments to create the mutant ΔpilA D. vulgaris, …

Chromium and uranium are highly toxic metals that contaminate many natural environments.
We investigated their mechanisms of toxicity under anaerobic conditions using nitrate-…

The reversible two-electron interconversion of formate and CO 2 is catalyzed by both nonmetallo-
and metallo-formate dehydrogenases (FDHs). The latter group comprises molybdenum…

Adeno-Associated Virus is the leading vector for gene therapy. Although it is the vector for
all in vivo gene therapies approved for clinical use by the US Food and Drug Administration, …