Synthetic biology relies on the manufacture of large and complex DNA constructs from
libraries of genetic parts. Golden Gate and other Type IIS restriction enzyme-dependent DNA …

Single-stranded DNA molecules (ssDNA) annealed to an RNA splint are notoriously poor
substrates for DNA ligases. Herein we report the unexpectedly efficient ligation of RNA-…

Large DNA constructs (>10 kb) are invaluable tools for genetic engineering and the
development of therapeutics. However, the manufacture of these constructs is laborious, often …

DNA assembly is an integral part of modern synthetic biology, as intricate genetic
engineering projects require robust molecular cloning workflows. Golden Gate assembly is a …

Ribonucleotide reductases (RNRs) catalyze the conversion of nucleotides to deoxynucleotides
in all organisms. The class I RNRs are composed of two subunits, α and β, with proposed …

DNA ligases, critical enzymes for in vivo genome maintenance and modern molecular
biology, catalyze the joining of adjacent 3′-OH and 5′-phosphorylated ends in DNA. To …

DNA ligases have broad application in molecular biology, from traditional cloning methods to
modern synthetic biology and molecular diagnostics protocols. Ligation-based detection of …

Small RNAs are important regulators of gene expression and are involved in human
development and disease. Next generation sequencing (NGS) allows for scalable, genome-wide …

DNA ligases are key enzymes in molecular and synthetic biology that catalyze the joining of
breaks in duplex DNA and the end-joining of DNA fragments. Ligation fidelity (discrimination …

Melioidosis is an emerging infectious disease caused by Burkholderia pseudomallei and is
associated with high morbidity and mortality rates in endemic areas. Antibiotic treatment is …