CRISPR/Cas9 has revolutionized our ability to engineer genomes and conduct genome-wide
screens in human cells 1 , 2 – 3 . Whereas some cell types are amenable to genome …

The evolutionarily conserved serine-threonine kinase mammalian target of rapamycin (mTOR)
plays a critical role in regulating many pathophysiological processes. Functional …

The RhoGDI proteins serve as key multifunctional regulators of Rho family GTP-binding
proteins. The 2.6 Å X-ray crystallographic structure of the Cdc42/RhoGDI complex reveals two …

Elucidation of the mutational landscape of human cancer has progressed rapidly and been
accompanied by the development of therapeutics targeting mutant oncogenes. However, a …

The repair outcomes at site-specific DNA double-strand breaks (DSBs) generated by the
RNA-guided DNA endonuclease Cas9 determine how gene function is altered. Despite the …

Defects in epigenetic regulation play a fundamental role in the development of cancer, and
epigenetic regulators have recently emerged as promising therapeutic candidates. We …

5-Methylthioadenosine phosphorylase (MTAP) is a key enzyme in the methionine salvage
pathway. The MTAP gene is frequently deleted in human cancers because of its chromosomal …

Macroautophagy is a key stress-response pathway that can suppress or promote tumorigenesis
depending on the cellular context. Notably, Kirsten rat sarcoma (KRAS)-driven tumors …

The metabolism of glucose and glutamine, primary carbon sources utilized by mitochondria
to generate energy and macromolecules for cell growth, is directly regulated by mTORC1. …

The Rho-related small GTP-binding protein Cdc42 has a low intrinsic GTPase activity that is
significantly enhanced by its specific GTPase-activating protein, Cdc42GAP. In this report, …