Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated
transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes …

Aging is the main risk factor for Alzheimer’s disease (AD); being associated with conspicuous
changes on microglia activation. Aged microglia exhibit an increased expression of …

We uncovered the diversity of non-canonical splice sites at the human transcriptome using
deep transcriptome profiling. We mapped a total of 3.7 billion human RNA-seq reads and …

Small RNAs play a crucial role in genome defense against transposable elements and guide
Argonaute proteins to nascent RNA transcripts to induce co-transcriptional gene silencing. …

Alternative DNA conformations, termed non-B DNA structures, can affect transcription, but
the underlying mechanisms and their functional impact have not been systematically …

Even though the functional role of mRNA molecules is primarily decided by the nucleotide
sequence, several properties are determined by secondary structure conformations. …

Alternative splicing is central to metazoan gene regulation, but the regulatory mechanisms
are incompletely understood. Here, we show that G-quadruplex (G4) motifs are enriched ~3-…

Single traumatic events that elicit an exaggerated stress response can lead to the development
of neuropsychiatric conditions. Rodent studies suggested germline RNA as a mediator of …

Stochastic gene expression leads to inherent variability in expression outcomes even in
isogenic single-celled organisms grown in the same environment. The Drop-Seq technology …

Background Microexons, exons that are ≤ 30 nucleotides, are a highly conserved and
dynamically regulated set of cassette exons. They have key roles in nervous system development …