DNA sequence information underpins genetic research, enabling discoveries of important
biological or medical benefit. Sequencing projects have traditionally used long (400–800 …

Background Next generation sequencing (NGS) technology has revolutionized genomic and
genetic research. The pace of change in this area is rapid with three major new sequencing …

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful
means to dissect genetic variation and its effect on gene expression. Here we describe G&T-…

High-throughput sequencing technology enables population-level surveys of human genomic
variation. Here, we examine the joint allele frequency distributions across continental …

Droplet-based single-cell RNA-seq has emerged as a powerful technique for massively parallel
cellular profiling. While this approach offers the exciting promise to deconvolute cellular …

Background Massively parallel sequencing technology is revolutionizing approaches to
genomic and genetic research. Since its advent, the scale and efficiency of Next-Generation …

PCR amplification introduces bias into Illumina sequencing libraries 1. Although amplification-free
library preparation solves this, micrograms of starting material are usually required. …

The cost of whole-genome sequencing (WGS) is decreasing rapidly as next-generation
sequencing technology continues to advance, and the prospect of making WGS available for …

Background A minor but significant fraction of samples subjected to next-generation sequencing
methods are either mixed-up or cross-contaminated. These events can lead to false or …

… Swerdlow and Gesteland [20] described DNA sequence analysis by use of capillary
polyacrylamide … Swerdlow and R. Gesteland, … Swerdlow, S. Wu, H. Harke and NJ Dovichi, J. …