High parameter imaging is an important tool in the life sciences for both discovery and
healthcare applications. Imaging Mass Cytometry (IMC) and Multiplexed Ion Beam Imaging (MIBI) …

Motivation High parameter histological techniques have allowed for the identification of a
variety of distinct cell types within an image, providing a comprehensive overview of the tissue …

Langerhans cells (LCs) situated in stratified squamous epithelium of the skin and mucosal
tissue are amongst the first cells that sexually transmitted pathogens encounter during …

… 96 h at a ratio of 2 T cells:1 DC and co-cultured for a further 72 h … h and co-cultured for a
further 96 h and the number of infected T cells was determined by flow cytometry. Infection at 2 h …

… C From a separate donor CD14 + CD1c − cells and CD14 + CD1c + cells were FACS isolated
following collagenase digestion and cultured for 0 h (unfilled, dotted histogram) and 24 h (…

… To isolate cells via spontaneous migration, the tissue was then cultured for 48 h in media
containing 10% FCS, 50 U/ml DNase I (F. Hoffmann-La Roche), and 25 µg/ml gentamicin. For …

… (TGFb) within 72 h7 and dermal DCs can express CD207,8 … Furthermore, MNPs rapidly
become activated within 2 h of … are likely to occur sooner than 2 h. Therefore, caution is needed …

Although HIV infection inhibits interferon responses in its target cells in vitro, interferon
signatures can be detected in vivo soon after sexual transmission, mainly attributed to …

The initial immune response to HIV determines transmission. However, due to technical
limitations we still do not have a comparative map of early mucosal transmission events. By …

The human intestine contains numerous mononuclear phagocytes (MNP), including subsets
of conventional dendritic cells (cDC), macrophages (Mf) and monocytes, each playing their …