Despite our rapidly growing knowledge about the human genome, we do not know all of the
genes required for some of the most basic functions of life. To start to fill this gap we …

Hepatitis C virus (HCV) is a major causative agent of chronic liver disease in humans. To gain
insight into host factor requirements for HCV replication, we performed a siRNA screen of …

… We imaged cells growing on scrambled siRNA every 30 min for 44 h starting 20 h after seeding…
By starting the imaging 20 h after plating cells on siRNA microarrays, the total observation …

The complete sequence of 57 kb of the human HPRT locus has been determined using
automated fluorescent DNA sequencing. The strategy employed increasingly directed …

The complete DNA sequence of the yeast Saccharomyces cerevisiae chromosome XI has
been determined. In addition to a compact arrangement of potential protein coding sequences…

… (H) HeLa cells transfected with indicated siRNAs stained with Filipin (images) and
counterstained for lysosomal protein Lamp1 (data not shown). Filipin signal intensities from …

… For time-lapse microscopy we typically incubate them for 20 h. For time-lapse microscopy
replace the medium 20 h after cell seeding with 5 ml CO 2 -independent growth medium just …

… (a) Overview of the numbers of genes having an effect on the distribution or appearance of
either the COPII coat, COPI coat or the Golgi matrix protein GM130 after 50 h downregulation, …

… After 10 h incubation at 37C the fluorescence signal of the expressed GFP signal can be
visualized. On the automatic scanning platform a motorized stage and motorized z-stepper …

… Parsons is funded by a Royal Society University Research Fellowship, H. Erfle and J. Beneke
by the CellNetworksCluster of Excellence (EXC81), J. Onuffer, WA Lim and TJ Mitchison …