… Nuclear extracts from HeLa cells (F and G) or from MEFs (H) were used for IP with the indicated
antibodies (IP Ab… WB signals were quantified and presented as percent of total input (H). …

… C for 1 h and finally 100% paraffin at 62 C for 2 h. The blocks containing embedded embryos
were cut into 15-μm sections, and the sections were dewaxed and viewed by microscopy. …

Background Cavefish populations belonging to the Mexican tetra species Astyanax
mexicanus are outstanding models to study the tempo and mode of adaptation to a radical …

… (H) Quantification of the size of 5HT neuronal groups in SF and CF. The surface area of the
immunolabeled region was measured. (I) HPLC measurement of 5HT in the anterior part and …

… Indeed, at 28C embryos progress from shield to bud stage in 2.5 h, which normally takes 4
h at 23C (data not shown). However, at this temperature the survival rate is low, and as such, …

Astyanax mexicanus, a teleost species with surface dwelling (surface fish) and cave adapted
(cavefish) morphs, is an important model system in evolutionary developmental biology (…

… The first peroxydase conjugate was inactivated by incubation in 2% H 2 O 2 for 1 hour.
Embryos were washed in PBST and incubated with the second antibody (anti-digoxygenin-POD, …

… The collected fish were placed in a 61-cm × 61-cm × 15-cm inflatable pool and were left for
an acclimatization period of 48 h. Two 50-ml syringes were attached to a stationary tripod and …

… Deprenyl (10 μM) was added during 5 h before the sacrifice of the animals. … 2-ml scintillation
cocktail overnight with agitation before counting 24 h later (the recovery of this method was …

Natural variations in sensory systems constitute adaptive responses to the environment.
Here, we compared sensory placode development in the blind cave-adapted morph and the …