Site-specific protein labeling with synthetic dyes is an emerging technique for live cell imaging.
A protein or peptide tag fused to the protein of interest provides the means for attachment …

Protein kinase G of Mycobacterium tuberculosis has been implicated in virulence and in
regulation of glutamate metabolism. Here we show that this kinase undergoes a pattern of …

… to equimolar amounts of H-HadB and HadC, and the second one corresponded to H-HadB
alone. A similar chromatogram was obtained with the fractions containing H-HadA and HadB, …

Sensing and response to changes in nutrient availability are essential for the lifestyle of
environmental and pathogenic bacteria. Serine/threonine protein kinase G (PknG) is required for …

The ketoreductase (KR) domains eryKR 1 and eryKR 2 from the erythromycin-producing
polyketide synthase (PKS) reduce 3-ketoacyl-thioester intermediates with opposite …

… Amide proton exchange was monitored by acquiring successive 15 N-HSQC spectra after
dissolution of lyophilized protein in 100% 2 H 2 O containing 20 mM sodium acetate (pH 5.8) …

The α-ketoglutarate dehydrogenase (KDH) complex is a major regulatory point of aerobic
energy metabolism. Mycobacterium tuberculosis was reported to lack KDH activity, and the …

… RPMI with or without ATc was replaced every 48 h. For 8 days, every 24 h, starting from 90
min after the initial washes, the medium was removed from three wells, and then intracellular …

Objective: The aim of this study was to highlight the vulnerability of the aging brain to surgery
and anesthesia, examine postoperative cognitive outcomes, and recommend possible …

Signaling by serine/threonine phosphorylation controls diverse processes in bacteria, and
identification of the stimuli that activate protein kinases is an outstanding question in the field. …