Practical considerations for quantitative light sheet fluorescence microscopy
… f–h, The resulting coregistration of neighboring tiles (f) shows better uniformity from the
center (g) to the periphery (h) of the overlap region, ensuring a better stitch and fusion. i, …
center (g) to the periphery (h) of the overlap region, ensuring a better stitch and fusion. i, …
Neural activity triggers neuronal oxidative metabolism followed by astrocytic glycolysis
KA Kasischke, HD Vishwasrao, PJ Fisher, WR Zipfel… - Science, 2004 - science.org
We have found that two-photon fluorescence imaging of nicotinamide adenine dinucleotide (NADH)
provides the sensitivity and spatial three-dimensional resolution to resolve …
provides the sensitivity and spatial three-dimensional resolution to resolve …
[HTML][HTML] Conformational Dependence of Intracellular NADH on Metabolic State Revealed by Associated Fluorescence Anisotropy*♦
HD Vishwasrao, AA Heikal, KA Kasischke… - Journal of Biological …, 2005 - ASBMB
Global analysis of fluorescence and associated anisotropy decays of intrinsic tissue
fluorescence offers a sensitive and non-invasive probe of the metabolically critical free/enzyme-…
fluorescence offers a sensitive and non-invasive probe of the metabolically critical free/enzyme-…
Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy
…, KA Kasischke, HD Vishwasrao… - Proceedings of the …, 2003 - National Acad Sciences
Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function
of neuronal processes. Here, polarized MT structures are selectively imaged with second-…
of neuronal processes. Here, polarized MT structures are selectively imaged with second-…
[HTML][HTML] Albumin/vaccine nanocomplexes that assemble in vivo for combination cancer immunotherapy
… b Upper: representative coronal (coro) PET images showing FVB mice at 6 h post sc …
Lower: representative transverse (trans) PET images showing LN delivery of MH 2 C at 6 h post …
Lower: representative transverse (trans) PET images showing LN delivery of MH 2 C at 6 h post …
Silica nanoparticle architecture determines radiative properties of encapsulated fluorophores
Silica nanoparticles with embedded fluorescent molecules are used in a variety of applications
requiring the observation of single nanoprobes. We describe a class of fluorescent, core–…
requiring the observation of single nanoprobes. We describe a class of fluorescent, core–…
Detection of antigen interactions ex vivo by proximity ligation assay: endogenous dopamine D2-adenosine A2A receptor complexes in the striatum
…, E Urizar, RA Piskorowski, HD Vishwasrao… - …, 2011 - Future Science
… We thank H. Thomas Lee for the generous gift of the A2A KO mice and Eleanor Simpson
and Mahalaxmi Aburi for their helpful comments on the manuscript. This work was supported in …
and Mahalaxmi Aburi for their helpful comments on the manuscript. This work was supported in …
Blinking and nonradiant dark fraction of water-soluble quantum dots in aqueous solution
Water-soluble quantum dots (qdots) are now being used in life sciences research to take
advantage of their bright, easily excited fluorescence and high photostability. Although the …
advantage of their bright, easily excited fluorescence and high photostability. Although the …
[HTML][HTML] Intertwining DNA-RNA nanocapsules loaded with tumor neoantigens as synergistic nanovaccines for cancer immunotherapy
… (FITC) L complexes for a total of 6, 24, and 48 h before confocal microscopy observation.
For 24-h and 48-h points, cells were washed at 14 h after adding iDR-NCs/CSIINFEK (FITC) L, …
For 24-h and 48-h points, cells were washed at 14 h after adding iDR-NCs/CSIINFEK (FITC) L, …
Rapid image deconvolution and multiview fusion for optical microscopy
…, D Green, A Beiriger, J Chen, H Vishwasrao… - Nature …, 2020 - nature.com
The contrast and resolution of images obtained with optical microscopes can be improved
by deconvolution and computational fusion of multiple views of the same sample, but these …
by deconvolution and computational fusion of multiple views of the same sample, but these …