Full-length RNA sequencing (RNA-Seq) has been applied to bulk tissue, cell lines and sorted
cells to characterize transcriptomes 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , but applying this …

Synapses are fundamental information-processing units of the brain, and synaptic dysregulation
is central to many brain disorders ("synaptopathies"). However, systematic annotation of …

Personal transcriptomes in which all of an individual’s genetic variants (eg, single nucleotide
variants) and transcript isoforms (transcription start sites, splice sites, and polyA sites) are …

Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared
to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes …

Eukaryotic cells make many types of primary and processed RNAs that are found either in
specific subcellular compartments or throughout the cells. A complete catalogue of these …

Splicing remains an incompletely understood process. Recent findings suggest that
chromatin structure participates in its regulation. Here, we analyze the RNA from subcellular …

The translation of personal genomics to precision medicine depends on the accurate
interpretation of the multitude of genetic variants observed for each individual. However, even …

Understanding transcriptome complexity is crucial for understanding human biology and
disease. Technologies such as Synthetic long-read RNA sequencing (SLR-RNA-seq) delivered …

RNA-sequencing (RNA-seq) is an essential technique for transcriptome studies, hundreds
of analysis tools have been developed since it was debuted. Although recent efforts have …

Splicing varies across brain regions, but the single-cell resolution of regional variation is
unclear. We present a single-cell investigation of differential isoform expression (DIE) between …