[PDF][PDF] A single CRISPR-Cas9 deletion strategy that targets the majority of DMD patients restores dystrophin function in hiPSC-derived muscle cells

CS Young, MR Hicks, NV Ermolova, H Nakano, M Jan… - Cell stem cell, 2016 - cell.com
Mutations in DMD disrupt the reading frame, prevent dystrophin translation, and cause
Duchenne muscular dystrophy (DMD). Here we describe a CRISPR/Cas9 platform applicable to …

[HTML][HTML] Haemogenic endocardium contributes to transient definitive haematopoiesis

H Nakano, X Liu, A Arshi, Y Nakashima… - Nature …, 2013 - nature.com
Haematopoietic cells arise from spatiotemporally restricted domains in the developing
embryo. Although studies of non-mammalian animal and in vitro embryonic stem cell models …

[HTML][HTML] Scl represses cardiomyogenesis in prospective hemogenic endothelium and endocardium

…, A Montel-Hagen, R Sasidharan, H Nakano… - Cell, 2012 - cell.com
Endothelium in embryonic hematopoietic tissues generates hematopoietic stem/progenitor
cells; however, it is unknown how its unique potential is specified. We show that transcription …

Glucose inhibits cardiac muscle maturation through nucleotide biosynthesis

H Nakano, I Minami, D Braas, H Pappoe, X Wu… - Elife, 2017 - elifesciences.org
10.7554/eLife.29330.001 The heart switches its energy substrate from glucose to fatty acids
at birth, and maternal hyperglycemia is associated with congenital heart disease. However, …

Mitochondrial Ca2+ uptake by the voltage-dependent anion channel 2 regulates cardiac rhythmicity

…, HDG Fiji, K Wang, H Zhu, C Tian, B Lin, H Nakano… - Elife, 2015 - elifesciences.org
10.7554/eLife.04801.001 Tightly regulated Ca 2+ homeostasis is a prerequisite for proper
cardiac function. To dissect the regulatory network of cardiac Ca 2+ handling, we performed a …

Rigid microenvironments promote cardiac differentiation of mouse and human embryonic stem cells

A Arshi, Y Nakashima, H Nakano… - … and technology of …, 2013 - iopscience.iop.org
While adult heart muscle is the least regenerative of tissues, embryonic cardiomyocytes are
proliferative, with embryonic stem (ES) cells providing an endless reservoir. In addition to …

[HTML][HTML] GLUT1 overexpression enhances glucose metabolism and promotes neonatal heart regeneration

…, M Pellegrini, H Christofk, H Nakano, A Nakano - Scientific reports, 2021 - nature.com
The mammalian heart switches its main metabolic substrate from glucose to fatty acids
shortly after birth. This metabolic switch coincides with the loss of regenerative capacity in the …

[PDF][PDF] Endocardially derived macrophages are essential for valvular remodeling

…, T Hsiai, A Deb, B Zhou, H Nakano, A Nakano - Developmental cell, 2019 - cell.com
During mammalian embryogenesis, de novo hematopoiesis occurs transiently in multiple
anatomical sites including the yolk sac, dorsal aorta, and heart tube. A long-unanswered …

[HTML][HTML] Complete atrial-specific knockout of sodium-calcium exchange eliminates sinoatrial node pacemaker activity

…, S Alber, R Zhang, ST Lamp, X Ren, H Nakano… - PloS one, 2013 - journals.plos.org
The origin of sinoatrial node (SAN) pacemaker activity in the heart is controversial. The leading
candidates are diastolic depolarization by “funny” current (I f ) through HCN4 channels (…

Flow-induced protein kinase A–CREB pathway acts via BMP signaling to promote HSC emergence

PG Kim, H Nakano, PP Das, MJ Chen… - Journal of Experimental …, 2015 - rupress.org
Fluid shear stress promotes the emergence of hematopoietic stem cells (HSCs) in the aorta–gonad–mesonephros
(AGM) of the developing mouse embryo. We determined that the AGM …