Enhancing xylose utilization has been a major focus in Saccharomyces cerevisiae strain-engineering
efforts. The incentive for these studies arises from the need to use all sugars in the …

As the closest living relatives of animals, choanoflagellates offer unique insights into animal
origins and core mechanisms underlying animal cell biology. However, unlike traditional …

Despite the need for inducible promoters in strain development efforts, the majority of
engineering in Saccharomyces cerevisiae continues to rely on a few constitutively active or …

The role of yhjX, a predicted major facilitator superfamily protein, was examined in context of
E. coli response to 1,4-butanediol (1,4-BDO). E. coli DH1 and MG1655, two commonly used …

Choanoflagellates have the potential to be important experimental models due to their close
relationship to animals, ability to develop into multicellular ‘rosettes’ that resemble animal …

… Authors: Heather L, Szmidt-Middleton; Mario, Ouellet; Paul D, Adams; Jay D, Keasling;
Aindrila, Mukhopadhyay …

… We thank Heather Szmidt-Middleton for help with initial strain archiving and testing and
Margaret Brown and Tristan de Rond for other technical assistance and helpful discussions. …

… rosetta cells in a similar pattern as in animal epithelia (Booth, SzmidtMiddleton, and King,
2018). Of particular relevance for the origin of animal cell types is the recent ultrastructural …

… We thank the following people for insights and support that helped advance this work: Heather
Szmidt-Middleton, Laura Wetzel, Monika Sigg, Lily Helfrich, Arielle Woznica, Sabrina Sun, …

The evolution of animal diversity is strongly affected by the origin of novel cell and tissue types
and their interactions with each other. Understanding the evolution of cell types will shed …