Tissue homeostasis and regeneration are fueled by resident stem cells that have the capacity
to self-renew, and to generate all the differentiated cell types that characterize a particular …

BACKGROUND & AIMS: Wnt signaling regulates multiple aspects of intestinal physiology,
including stem cell maintenance. Paneth cells support stem cells by secreting Wnt, but little is …

Intestinal crypts display robust regeneration upon injury. The relatively rare secretory precursors
can replace lost stem cells, but it is unknown if the abundant enterocyte progenitors that …

Mammalian Wnt proteins are believed to act as short-range signals 1 , 2 , 3 , 4 , yet have not
been previously visualized in vivo. Self-renewal, proliferation and differentiation are …

Although Lgr5 + intestinal stem cells have been expanded in vitro as organoids, homogeneous
culture of these cells has not been possible thus far. Here we show that two small …

The study of gene function in endodermal epithelia such as of stomach, small intestine and
colon relies heavily on transgenic approaches. Establishing such animal models is laborious, …

Paneth cells (PCs) are terminally differentiated, highly specialized secretory cells located at
the base of the crypts of Lieberkühn in the small intestine. Besides their antimicrobial function…

Organoid technology allows the expansion of primary epithelial cells from normal and
diseased tissues, providing a unique model for human (patho)biology. In a three-dimensional …

Immunotherapy using chimeric antigen receptor ( CAR )‐engineered lymphocytes has shown
impressive results in leukemia. However, for solid tumors such as colorectal cancer ( CRC )…

Objective Cancer-associated fibroblasts (CAFs) influence the tumour microenvironment and
tumour growth. However, the role of CAFs in colorectal cancer (CRC) development is …