Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor
compartment and fuse with an acceptor compartment. Common principles of budding and fusion …

Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular
trafficking. We used an integrative approach, combining quantitative immunoblotting and …

Major structural changes occur in the spliceosome during its activation just before catalyzing
the splicing of pre–messenger RNAs (pre-mRNAs). Whereas changes in small nuclear …

Metazoan gene regulation often involves the pausing of RNA polymerase II (Pol II) in the
promoter-proximal region. Paused Pol II is stabilized by the protein complexes DRB sensitivity-…

Small interfering RNAs (siRNAs) are the mediators of mRNA degradation in the process of
RNA interference (RNAi). Here, we describe a human biochemical system that recapitulates …

RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs)
[1–4]. They reside in ribonucleoprotein complexes, which guide the cleavage of …

We developed a method, named GraFix, that considerably improves sample quality for
structure determination by single-particle electron cryomicroscopy (cryo-EM). GraFix uses a …

The structure, dynamic behavior, and spatial organization of microtubules are regulated by
microtubule-associated proteins. An important microtubule-associated protein is the protein …

N 6 ‐methyladenosine (m 6 A) is a highly dynamic RNA modification that has recently emerged
as a key regulator of gene expression. While many m 6 A modifications are installed by …

Elongation factor P (EF-P) is a translation factor of unknown function that has been implicated
in a great variety of cellular processes. Here, we show that EF-P prevents ribosome from …