We identify a role for the GDI-like solubilizing factor (GSF) PDEδ in modulating signalling
through Ras family G proteins by sustaining their dynamic distribution in cellular membranes. …

Our understanding of the plasma membrane, once viewed simply as a static barrier, has been
revolutionized to encompass a complex, dynamic organelle that integrates the cell with its …

Since the end of the 17th century, when Robert Hooke first coined the word “cell” and Antonie
van Leeuwenhoek first observed single-celled organisms, optical microscopy has been an …

The erbB/HER family of transmembrane receptor tyrosine kinases (RTKs) mediate cellular
responses to epidermal growth factor (EGF) and related ligands. We have imaged the early …

Extracellular stimuli are transduced inside the cell by posttranslational modifications (PTMs),
such as phosphorylation, of proteins in signaling networks. Insight into the structure of these …

The proto-oncogenic epidermal growth factor receptor (EGFR) is a tyrosine kinase whose
sensitivity to growth factors and signal duration determines cellular behavior. We resolve how …

Ubiquitin chains modify a major subset of the proteome, but detection of ubiquitin signaling
dynamics and localization is limited due to a lack of appropriate tools. Here, we employ …

The localization and signaling of S-palmitoylated peripheral membrane proteins is
sustained by an acylation cycle in which acyl protein thioesterases (APTs) depalmitoylate …

Fluorescence lifetime imaging microscopy (FLIM) can be used to quantify molecular reactions
in cells by detecting fluorescence resonance energy transfer (FRET). Confocal FLIM …

Smaug is a conserved translational regulator that binds numerous mRNAs, including nuclear
transcripts that encode mitochondrial enzymes. Smaug orthologs form cytosolic membrane-…