We introduce a PCR-based procedure for generating a gene disruption construct. This method
depends on DNA fragment fusion by the PCR technique and requires only two steps of …

Self-regulating systems often use robust oscillatory circuits. One such system controls the
chemotactic signaling mechanism of Dictyostelium, where pulses of adenosine 3′,5′-…

Switching between attractive and repulsive migration in cell movement in response to
extracellular guidance cues has been found in various cell types and is an important cellular …

A distinct feature of development in the simple eukaryote Dictyostelium discoideum is an
aggregative transition from a unicellular to a multicellular phase. Using genome-wide …

Solitons have been observed in various physical phenomena. Here, we show that the
distinct characteristics of solitons are present in the mass cell movement of non-chemotactic …

Chloroplasts have evolved from a cyanobacterial endosymbiont and been retained for more
than 1 billion years by coordinated chloroplast division in multiplying eukaryotic cells. …

Conventional myosin functions universally as a generator of motive force in eukaryotic cells.
Analysis of mutants of the microorganism Dictyostelium discoideum revealed that myosin …

Folic acid and cAMP are chemoattractants in Dictyostelium discoideum, which bind to different
surface receptors. The signal is transduced from the receptors via different G proteins into …

Biophysical mechanisms underlying collective cell migration of eukaryotic cells have been
studied extensively in recent years. One mechanism that induces cells to correlate their …

Actin‐depolymerizing factor (ADF)/cofilin is widely expressed in eukaryotes and plays a
central role in reorganizing the actin cytoskeleton by disassembling actin filaments. The ADF‐…