DNA methylation is extensively reprogrammed during the early phases of mammalian
development, yet genomic targets of this process are largely unknown. We optimized methylated …

A fundamental question in cell and developmental biology is how epithelial cells construct
the diffusion barrier allowing them to separate different body compartments. Formation of tight …

RNA interference (RNAi) is a mechanism by which double-stranded RNAs (dsRNAs) suppress
specific transcripts in a sequence-dependent manner. dsRNAs are processed by Dicer to …

Trophoblast cells play an essential role in the interactions between the fetus and mother.
Mouse trophoblast stem (TS) cells have been derived and used as the best in vitro model for …

Three integral membrane proteins, clau- din-1, -2, and occludin, are known to be components
of tight junction (TJ) strands. To examine their ability to form TJ strands, their cDNAs were …

Human in vitro gametogenesis may transform reproductive medicine. Human pluripotent stem
cells (hPSCs) have been induced into primordial germ cell–like cells (hPGCLCs); however…

Zygotic epigenetic reprogramming entails genome-wide DNA demethylation that is accompanied
by Tet methylcytosine dioxygenase 3 (Tet3)-driven oxidation of 5-methylcytosine (5mC…

Tight junctions are well-developed between adjacent endothelial cells of blood vessels in
the central nervous system, and play a central role in establishing the blood-brain barrier (BBB)…

Imprinted genes are epigenetically marked during gametogenesis so that they are
exclusively expressed from either the paternal or the maternal allele in offspring 1 . Imprinting …

Occludin is an integral membrane protein with four transmembrane domains that is exclusively
localized at tight junction (TJ) strands. Here, we describe the generation and analysis of …