The type VII secretion system of Staphylococcus aureus secretes a nuclease toxin that targets competitor bacteria

Z Cao, MG Casabona, H Kneuper, JD Chalmers… - Nature …, 2016 - nature.com
The type VII protein secretion system (T7SS) plays a critical role in the virulence of human
pathogens including Mycobacterium tuberculosis and Staphylococcus aureus. Here, we report …

Heterogeneity in ess transcriptional organization and variable contribution of the Ess/Type VII protein secretion system to virulence across closely related S …

H Kneuper, ZP Cao, KB Twomey… - Molecular …, 2014 - Wiley Online Library
The Type VII protein secretion system, found in G ram‐positive bacteria, secretes small
proteins, containing a conserved W ‐x‐ G amino acid sequence motif, to the growth medium. S …

Plasticity of the PAS domain and a potential role for signal transduction in the histidine kinase DcuS

M Etzkorn, H Kneuper, P Dünnwald, V Vijayan… - Nature structural & …, 2008 - nature.com
The mechanistic understanding of how membrane-embedded sensor kinases recognize
signals and regulate kinase activity is currently limited. Here we report structure-function …

A membrane-depolarizing toxin substrate of the Staphylococcus aureus type VII secretion system mediates intraspecies competition

…, JD Chalmers, Z Cao, H Kneuper… - Proceedings of the …, 2020 - National Acad Sciences
The type VII protein secretion system (T7SS) is conserved across Staphylococcus aureus
strains and plays important roles in virulence and interbacterial competition. To date, only one …

Molecular dissection of TatC defines critical regions essential for protein transport and a TatB–TatC contact site

H Kneuper, B Maldonado, F Jäger… - Molecular …, 2012 - Wiley Online Library
The twin arginine transport (Tat) system transports folded proteins across the prokaryotic
cytoplasmic membrane and the plant thylakoid membrane. TatC is the largest and most …

EssC: domain structures inform on the elusive translocation channel in the Type VII secretion system

…, WMAV Ng, JJ Money, PK Fyfe, H Kneuper… - Biochemical …, 2016 - portlandpress.com
The membrane-bound protein EssC is an integral component of the bacterial Type VII secretion
system (T7SS), which is a determinant of virulence in important Gram-positive pathogens…

[HTML][HTML] The nature of the stimulus and of the fumarate binding site of the fumarate sensor DcuS of Escherichia coli

H Kneuper, IG Janausch, V Vijayan… - Journal of Biological …, 2005 - ASBMB
DcuS is a membrane-associated sensory histidine kinase of Escherichia coli specific for C 4
-dicarboxylates. The nature of the stimulus and its structural prerequisites were determined …

Discovery of an Allosteric Inhibitor Binding Site in 3-Oxo-acyl-ACP Reductase from Pseudomonas aeruginosa

…, L Moynie, R Schnell, S Schach, H Kneuper… - ACS chemical …, 2013 - ACS Publications
3-Oxo-acyl-acyl carrier protein (ACP) reductase (FabG) plays a key role in the bacterial fatty
acid synthesis II system in pathogenic microorganisms, which has been recognized as a …

A signal sequence suppressor mutant that stabilizes an assembled state of the twin arginine translocase

Q Huang, F Alcock, H Kneuper… - Proceedings of the …, 2017 - National Acad Sciences
The twin-arginine protein translocation (Tat) system mediates transport of folded proteins
across the cytoplasmic membrane of bacteria and the thylakoid membrane of chloroplasts. The …

The TatC component of the twin‐arginine protein translocase functions as an obligate oligomer

…, J Habersetzer, F Alcock, H Kneuper… - Molecular …, 2015 - Wiley Online Library
The Tat protein export system translocates folded proteins across the bacterial cytoplasmic
membrane and the plant thylakoid membrane. The Tat system in E scherichia coli is …