To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi
Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: …

The CRISPR/Cas9 system has recently emerged as a powerful tool for functional genomic
studies in Drosophila melanogaster. However, single-guide RNA (sgRNA) parameters …

Background: A miniature spine-mounted robot has recently been introduced to further improve
the accuracy of pedicle screw placement in spine surgery. However, the differences in …

Being relatively simple and practical, Drosophila transgenic RNAi is the technique of top
priority choice to quickly study genes with pleiotropic functions. However, drawbacks have …

CRISPR/Cas9-based transcriptional activation (CRISPRa) has recently emerged as a
powerful and scalable technique for systematic overexpression genetic analysis in Drosophila …

Recent studies of the Cas9/sgRNA system in Drosophila melanogaster genome editing
have opened new opportunities to generate site-specific mutant collections in a high-throughput …

The last couple of years have witnessed an explosion in development of CRISPR-based
genome editing technologies in cell lines as well as in model organisms. In this review, we …

Epigenetics plays critical roles in controlling stem cell self-renewal and differentiation. Histone
H1 is one of the most critical chromatin regulators, but its role in adult stem cell regulation …

Adenosine-to-inosine RNA editing, catalyzed by adenosine deaminase acting on RNA (ADAR)
enzymes, alters RNA sequences from those encoded by DNA. These editing events are …

… Two reviewers (Fei-Hu Li and Huan-Huan Qiao) evaluated and extracted data independently;
discrepancies were resolved through discussion until a consensus was reached. The …