User profiles for Hugh Olsen
Hugh OlsenProject Scientist, University of California, Santa Cruz Verified email at soe.ucsc.edu Cited by 9383 |
[HTML][HTML] Nanopore sequencing and assembly of a human genome with ultra-long reads
We report the sequencing and assembly of a reference genome for the human GM12878
Utah/Ceph cell line using the MinION (Oxford Nanopore Technologies) nanopore sequencer. …
Utah/Ceph cell line using the MinION (Oxford Nanopore Technologies) nanopore sequencer. …
[HTML][HTML] The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community
Nanopore DNA strand sequencing has emerged as a competitive, portable technology. Reads
exceeding 150 kilobases have been achieved, as have in-field detection and analysis of …
exceeding 150 kilobases have been achieved, as have in-field detection and analysis of …
[HTML][HTML] A draft human pangenome reference
Here the Human Pangenome Reference Consortium presents a first draft of the human
pangenome reference. The pangenome contains 47 phased, diploid assemblies from a cohort of …
pangenome reference. The pangenome contains 47 phased, diploid assemblies from a cohort of …
Improved data analysis for the MinION nanopore sequencer
Speed, single-base sensitivity and long read lengths make nanopores a promising technology
for high-throughput sequencing. We evaluated and optimized the performance of the …
for high-throughput sequencing. We evaluated and optimized the performance of the …
[HTML][HTML] Nanopore sequencing and the Shasta toolkit enable efficient de novo assembly of eleven human genomes
De novo assembly of a human genome using nanopore long-read sequences has been
reported, but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable …
reported, but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable …
Nanopore native RNA sequencing of a human poly (A) transcriptome
High-throughput complementary DNA sequencing technologies have advanced our
understanding of transcriptome complexity and regulation. However, these methods lose …
understanding of transcriptome complexity and regulation. However, these methods lose …
Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel
RNA and DNA strands produce ionic current signatures when driven through an α-hemolysin
channel by an applied voltage. Here we combine this nanopore detector with a support …
channel by an applied voltage. Here we combine this nanopore detector with a support …
The complete sequence of a human Y chromosome
…, A Mikheenko, KM Munson, TD Murphy, HE Olsen… - Nature, 2023 - nature.com
The human Y chromosome has been notoriously difficult to sequence and assemble
because of its complex repeat structure that includes long palindromes, tandem repeats and …
because of its complex repeat structure that includes long palindromes, tandem repeats and …
Mapping DNA methylation with high-throughput nanopore sequencing
DNA chemical modifications regulate genomic function. We present a framework for mapping
cytosine and adenosine methylation with the Oxford Nanopore Technologies MinION …
cytosine and adenosine methylation with the Oxford Nanopore Technologies MinION …
[HTML][HTML] Semi-automated assembly of high-quality diploid human reference genomes
…, MW Mitchell, J McDaniel, F Nie, HE Olsen… - Nature, 2022 - nature.com
The current human reference genome, GRCh38, represents over 20 years of effort to generate
a high-quality assembly, which has benefitted society 1 , 2 . However, it still has many …
a high-quality assembly, which has benefitted society 1 , 2 . However, it still has many …