INTRODUCTION Organisms live by means of the complex, dynamic, three-dimensional (3D)
interplay between millions of components, from the molecular to the multicellular. …

Sharply delineated domains of cell types arise in developing tissues under instruction of
inductive signal (morphogen) gradients, which specify distinct cell fates at different signal levels. …

Epithelial cells acquire functionally important shapes (eg, squamous, cuboidal, columnar)
during development. Here, we combine theory, quantitative imaging, and perturbations to …

The quantification of cell shape, cell migration, and cell rearrangements is important for
addressing classical questions in developmental biology such as patterning and tissue …

Introns may affect gene expression by increasing the time required to transcribe the gene.
One way for extended transcription times to affect the behavior of a gene expression program …

The time taken to transcribe most metazoan genes is significant because of the substantial
length of introns. Developmentally regulated gene networks, where timing and dynamic …

Forward genetic screens in zebrafish have identified >9000 mutants, many of which are potential
disease models. Most mutants remain molecularly uncharacterized because of the high …

Rapid advances in microscopy and genetic labeling strategies have created new opportunities
for time-lapse imaging of embryonic development. However, methods for immobilizing …

10.7554/eLife.39596.001 Animals make organs of precise size, shape, and symmetry but
how developing embryos do this is largely unknown. Here, we combine quantitative imaging, …

Background Transcription by RNA polymerase II is regulated at many steps including
initiation, promoter release, elongation and termination. Accumulation of RNA polymerase II at …