Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for
the identification of protein-protein interactions. However, for any given protein of interest, …

Sirtuins (SIRTs) are critical enzymes that govern genome regulation, metabolism, and aging.
Despite conserved deacetylase domains, mitochondrial SIRT4 and SIRT5 have little to no …

Protein complexes mediate the majority of cellular processes. Knowledge of the localization
and composition of such complexes provides key insights into their functions. Although …

We present a quantitative model to demonstrate that coclustering multiple enzymes into
compact agglomerates accelerates the processing of intermediates, yielding the same efficiency …

The eukaryotic cell is compartmentalized into subcellular niches, including membrane-bound
and membrane-less organelles. Proteins localize to these niches to fulfil their function, …

The complexity of intracellular signalling requires both a diversity of molecular players and
the sequestration of activity to unique compartments within the cell. Recent findings on the …

The Human Proteome Organization (HUPO) launched the Human Proteome Project (HPP)
in 2010, creating an international framework for global collaboration, data sharing, quality …

The incorporation of histone H3 variants has been implicated in the epigenetic memory of
cellular state. Using genome editing with zinc-finger nucleases to tag endogenous H3.3, we …

Autophagy is a highly regulated cellular mechanism for the bulk degradation of cytoplasmic
contents. It has been implicated in a variety of physiological and pathological conditions …

Detection of pathogenic nucleic acids is essential for mammalian innate immunity. IFN-inducible
protein IFI16 has emerged as a critical sensor for detecting pathogenic DNA, stimulating …