The first in vivo measurements of a protein diffusion coefficient versus cytoplasmic biopolymer
volume fraction are presented. Fluorescence recovery after photobleaching yields the …

Understanding the interaction between soluble factors and cells in the cellular microenvironment
is critical to understanding a wide range of diseases. Microchannel culture systems …

Facile diffusion of globular proteins within a cytoplasm that is dense with biopolymers is
essential to normal cellular biochemical activity and growth. Remarkably, Escherichia coli grows …

In this work, we obtain the data needed to predict chemical interactions of polyethylene glycols
(PEGs) and glycerol with proteins and related organic compounds and thereby interpret …

The physical and mechanical properties of the cell envelope of Escherichia coli are poorly
understood. We use fluorescence recovery after photobleaching to measure diffusion of …

E. coli single-stranded-DNA binding protein (EcSSB) displays nearest-neighbor (NN) and
non-nearest-neighbor (NNN)) cooperativity in binding ssDNA during genome maintenance. …

Small and large PEGs greatly increase chemical potentials of globular proteins (μ 2 ), thereby
favoring precipitation, crystallization, and protein–protein interactions that reduce water‐…

Many studies of specific protein-nucleic acid binding use short oligonucleotides or restriction
fragments, in part to minimize the potential for nonspecific binding of the protein. However, …

Transcription initiation is highly regulated by promoter sequence, transcription factors, and
ligands. All known transcription inhibitors, an important class of antibiotics, act in initiation. To …

Potassium glutamate (KGlu) is the primary Escherichia coli cytoplasmic salt. After sudden
osmotic upshift, cytoplasmic KGlu concentration increases, initially because of water efflux and …