We present a new method to measure absolute diffusion coefficients at nanomolar concentrations
with high precision. Based on a modified fluorescence correlation spectroscopy (FCS)‐…

Cytochrome aa 3 from Paracoccus denitrificans and cytochrome ba 3 from Thermus thermophilus,
two distinct members of the heme–copper oxidase superfamily, were immobilized on …

Exact knowledge of the numerical aperture is crucial in many applications using high-aperture
objectives such as confocal microscopy, optical trapping, or advanced sub-wavelength …

Stable capsid structures of viruses protect viral RNA while they also require controlled
disassembly for releasing the viral genome in the host cell. A detailed understanding of viral …

In order to monitor membrane–protein binding in lipid bilayers at physiological protein
concentrations, we employed the recently developed dual‐focus fluorescence correlation …

The diffusion of fluorescently labeled lipids in supported bilayers is studied using two
different methods: Z-scan fluorescence correlation spectroscopy (z-scan FCS) and two-focus …

Crystallization of membrane proteins is very laborious and time-consuming, yielding well
diffracting crystals in only a minority of projects. Therefore, a rapid and easy method is required …

Cytochrome c oxidase (CcO) is the terminal enzyme of the respiratory chain. This redox-driven
proton pump catalyzes the four-electron reduction of molecular oxygen to water, one of …

The four subunit (SU) aa 3 cytochrome c oxidase (CcO) from Paracoccus denitrificans is one
of the terminal enzymes of the respiratory chain. Its binuclear active center, residing in SU I, …

… Our results support the hypothesis by von der Hocht et al. [von der Hocht, I., et al. (2011) …
612 nm in the P N state that was first observed by von der Hocht et al. The P N state can also be …