The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can
be implemented flexibly in living cells and in vivo. However, the design of synthetic …

Organisms are defined by the information encoded in their genomes, and since the origin of
life this information has been encoded using a two-base-pair genetic alphabet (A–T and G–…

Bisulfite sequencing detects 5mC and 5hmC at single-base resolution. However, bisulfite
treatment damages DNA, which results in fragmentation, DNA loss, and biased sequencing …

The ability to specifically attach chemical probes to individual proteins represents a powerful
approach to the study and manipulation of protein function in living cells. It provides a simple…

10.7554/eLife.03970.001 Several different endocytic pathways have been proposed to
function in mammalian cells. Clathrin-coated pits are well defined, but the identity, mechanism …

R-loops are nucleic acid structures formed by an RNA:DNA hybrid and unpaired single-stranded
DNA that represent a source of genomic instability in mammalian cells 1 , 2 , 3 – 4 . …

Glucagon-like peptide-1 receptor (GLP-1R) activation promotes insulin secretion from
pancreatic beta cells, causes weight loss, and is an important pharmacological target in type 2 …

Filopodia are slender cellular protrusions that dynamically extend and retract to facilitate
directional cell migration, pathogen sensing, and cell-cell adhesion [1–4]. Each filopodium …

Diffraction-unlimited resolution provided by Stimulated Emission Depletion (STED) microscopy
allows for imaging cellular processes in living cells that are not visible by conventional …

Cytosine residues in mammalian DNA occur in five forms: cytosine (C), 5-methylcytosine (5mC),
5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). …