Robust heat shock induces eIF2α-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast …

T Grousl, P Ivanov, I Frydlová, P Vasicová… - Journal of cell …, 2009 - journals.biologists.com
Environmental stresses inducing translation arrest are accompanied by the deposition of
translational components into stress granules (SGs) serving as mRNA triage sites. It has …

[HTML][HTML] Heat Shock-Induced Accumulation of Translation Elongation and Termination Factors Precedes Assembly of Stress Granules in S. cerevisiae

T Grousl, P Ivanov, I Malcova, P Pompach, I Frydlova… - PloS one, 2013 - journals.plos.org
In response to severe environmental stresses eukaryotic cells shut down translation and
accumulate components of the translational machinery in stress granules (SGs). Since they …

[HTML][HTML] Cytotoxicity of the effector protein BteA was attenuated in Bordetella pertussis by insertion of an alanine residue

J Bayram, I Malcova, L Sinkovec, J Holubova… - PLoS …, 2020 - journals.plos.org
Bordetella bronchiseptica and Bordetella pertussis are closely related respiratory
pathogens that evolved from a common bacterial ancestor. While B. bronchiseptica has an …

BopN is a Gatekeeper of the Bordetella Type III Secretion System

…, L Bumba, T Prudnikova, I Malcova… - Microbiology …, 2023 - Am Soc Microbiol
The classical Bordetella species infect the respiratory tract of mammals. While B. bronchiseptica
causes rather chronic respiratory infections in a variety of mammals, the human-adapted …

New integrative modules for multicolor-protein labeling and live-cell imaging in Saccharomyces cerevisiae

I Malcova, M Farkasovsky, L Senohrabkova… - FEMS Yeast …, 2016 - academic.oup.com
Live-imaging analysis is performed in many laboratories all over the world. Various tools
have been developed to enable protein labeling either in plasmid or genomic context in live …

The Stationary-Phase Cells of Saccharomyces cerevisiae Display Dynamic Actin Filaments Required for Processes Extending Chronological Life Span

P Vasicova, R Lejskova, I Malcova… - Molecular and cellular …, 2015 - Taylor & Francis
Stationary-growth-phase Saccharomyces cerevisiae yeast cultures consist of nondividing
cells that undergo chronological aging. For their successful survival, the turnover of proteins …

[HTML][HTML] eIF3a destabilization and TDP-43 alter dynamics of heat-induced stress granules

I Malcova, L Senohrabkova, L Novakova… - International Journal of …, 2021 - mdpi.com
Stress granules (SGs) are membrane-less assemblies arising upon various stresses in
eukaryotic cells. They sequester mRNAs and proteins from stressful conditions and modulate …

Vps factors are required for efficient transcription elongation in budding yeast

…, DG Brickner, H Qiu, F Zhang, CM Wong, I Malcova… - Genetics, 2013 - academic.oup.com
There is increasing evidence that certain Vacuolar protein sorting (Vps) proteins, factors that
mediate vesicular protein trafficking, have additional roles in regulating transcription factors …

[HTML][HTML] Lipid binding by the N-terminal motif mediates plasma membrane localization of Bordetella effector protein BteA

I Malcova, L Bumba, F Uljanic, D Kuzmenko… - Journal of Biological …, 2021 - ASBMB
The respiratory pathogens Bordetella pertussis and Bordetella bronchiseptica employ a
type III secretion system (T3SS) to inject a 69-kDa BteA effector protein into host cells. This …

Deregulation of DSE1 Gene Expression Results in Aberrant Budding within the Birth Scar and Cell Wall Integrity Pathway Activation in Saccharomyces cerevisiae

I Frýdlová, I Malcová, P Vašicová, J Hašek - Eukaryotic Cell, 2009 - Am Soc Microbiol
Strains of Saccharomyces cerevisiae lacking Isw2, the catalytic subunit of the Isw2 chromatin
remodeling complex, show the mating type-independent activation of the cell wall integrity (…