Immunoglobulin (Ig) loci are selectively activated for transcription and rearrangement during
B lymphocyte development. Using fluorescence in situ hybridization, we show that Ig heavy (…

Polycomb and Trithorax group proteins encode the epigenetic memory of cellular positional
identity by establishing inheritable domains of repressive and active chromatin within the …

Mutations in genes involved in DNA methylation (DNAme; for example, TET2 and DNMT3A)
are frequently observed in hematological malignancies 1 , 2 – 3 and clonal hematopoiesis 4 …

Allelic exclusion of immunoglobulin genes ensures the expression of a single antibody
molecule in B cells through mostly unknown mechanisms. Large-scale contraction of the …

Polycomb repressive complex 2 (PRC2) maintains gene silencing by catalyzing methylation
of histone H3 at lysine 27 (H3K27me2/3) within chromatin. By designing a system whereby …

Productive rearrangement of the immunoglobulin heavy-chain locus triggers a major
developmental checkpoint that promotes limited clonal expansion of pre-B cells, thereby …

The cohesin complex (consisting of Rad21, Smc1a, Smc3, and Stag2 proteins) is critically
important for proper sister chromatid separation during mitosis. Mutations in the cohesin …

… We thank members of the Skok laboratory for helpful discussions and the NYU microscopy
core for technical support. PPR is a National Cancer Center and American Society of …

… Skok … Similar results were obtained with IL-7–stimulated primary bone marrow cultures
derived from RAG-1–deficient mice (JA Skok et al., unpublished data). … Skok …

Coordinated recombination of homologous antigen receptor loci is thought to be important
for allelic exclusion. Here we show that homologous immunoglobulin alleles pair in a stage-…