The differences between individual cells can have profound functional consequences, in
both unicellular and multicellular organisms. Recently developed single-cell mRNA-…

The development of high-throughput RNA sequencing (RNA-seq) at the single-cell level has
already led to profound new discoveries in biology, ranging from the identification of novel …

The recent boom in microfluidics and combinatorial indexing strategies, combined with low
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …

Ultra-high-throughput sequencing is emerging as an attractive alternative to microarrays for
genotyping, analysis of methylation patterns, and identification of transcription factor binding …

10.7554/eLife.27041.001 The recent advent of methods for high-throughput single-cell
molecular profiling has catalyzed a growing sense in the scientific community that the time is ripe …

Glioblastoma (GB) is the most common and aggressive primary brain malignancy, with poor
prognosis and a lack of effective therapeutic options. Accumulating evidence suggests that …

Large-scale single-cell RNA sequencing (scRNA-seq) data sets that are produced in
different laboratories and at different times contain batch effects that may compromise the …

Understanding the genetic mechanisms underlying natural variation in gene expression is a
central goal of both medical and evolutionary genetics, and studies of expression …

Abstract Analysis of human blood immune cells provides insights into the coordinated
response to viral infections such as severe acute respiratory syndrome coronavirus 2, which …

Single-cell RNA sequencing (scRNA-seq) is widely used to profile the transcriptome of
individual cells. This provides biological resolution that cannot be matched by bulk RNA …