User profiles for J. D. Boeke
 | Jef D. BoekeNYU Langone Health Verified email at nyulangone.org Cited by 72645 |
The biochemistry of sirtuins
…, C Wolberger, VL Schramm, JD Boeke - Annu. Rev …, 2006 - annualreviews.org
Sirtuins are a family of NAD + -dependent protein deacetylases widely distributed in all phyla
of life. Accumulating evidence indicates that sirtuins are important regulators of organism …
of life. Accumulating evidence indicates that sirtuins are important regulators of organism …
Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome …
We conducted a genome-wide survey of Saccharomyces cerevisiae retrotransposons and
identified a total of 331 insertions, including 217 Ty1, 34 Ty2, 41 Ty3, 32 Ty4, and 7 Ty5 …
identified a total of 331 insertions, including 217 Ty1, 34 Ty2, 41 Ty3, 32 Ty4, and 7 Ty5 …
Retrotransposons, endogenous retroviruses, and the evolution of retroelements
The retroelements are as diverse an assemblage of related molecular entities as can be
found anywhere. With the exception of the retroviruses themselves, retroelements are genetic …
found anywhere. With the exception of the retroviruses themselves, retroelements are genetic …
Functional profiling of the Saccharomyces cerevisiae genome
Determining the effect of gene deletion is a fundamental approach to understanding gene
function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype …
function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype …
Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis
The functions of many open reading frames (ORFs) identified in genome-sequencing projects
are unknown. New, whole-genome approaches are required to systematically determine …
are unknown. New, whole-genome approaches are required to systematically determine …
Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR‐mediated gene disruption and other …
… Cost, GC and Boeke, JD (1996). A useful colony colour phenotype associated with the yeast
… Sikorski, RS and Boeke, JD (1990). In vitro mutagenesis and plasmid shuffling: from cloned …
… Sikorski, RS and Boeke, JD (1990). In vitro mutagenesis and plasmid shuffling: from cloned …
A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
JD Boeke, F La Croute, GR Fink - Molecular and General Genetics MGG, 1984 - Springer
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive
selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3 + …
selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3 + …
[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics
JD Boeke, J Trueheart, G Natsoulis, GR Fink - Methods in enzymology, 1987 - Elsevier
5-FOA is an extremely useful reagent for the selection of Ura − cells amid a population of Ura
+ cells. The selection is effective in transformation and recombination studies where loss of …
+ cells. The selection is effective in transformation and recombination studies where loss of …
[HTML][HTML] Human L1 retrotransposon encodes a conserved endonuclease required for retrotransposition
Human L1 elements are highly abundant poly(A) (non-LTR) retrotransposons whose second
open reading frame (ORF2) encodes a reverse transcriptase (RT). We have identified an …
open reading frame (ORF2) encodes a reverse transcriptase (RT). We have identified an …
[HTML][HTML] High frequency retrotransposition in cultured mammalian cells
We previously isolated two human L1 elements (L1.2 and LRE2) as the progenitors of
disease-producing insertions. Here, we show these elements can actively retrotranspose in …
disease-producing insertions. Here, we show these elements can actively retrotranspose in …