User profiles for J. D. Buenrostro

Jason Buenrostro

Harvard University
Verified email at harvard.edu
Cited by 22991

Single-cell and single-molecule epigenomics to uncover genome regulation at unprecedented resolution

E Shema, BE Bernstein, JD Buenrostro - Nature genetics, 2019 - nature.com
Recent advances in single-cell and single-molecule epigenomic technologies now enable
the study of genome regulation and dynamics at unprecedented resolution. In this Perspective…

[PDF][PDF] Advances in chromatin and chromosome research: perspectives from multiple fields

AA Agbleke, A Amitai, JD Buenrostro, A Chakrabarti… - Molecular cell, 2020 - cell.com
Nucleosomes package genomic DNA into chromatin. By regulating DNA access for transcription,
replication, DNA repair, and epigenetic modification, chromatin forms the nexus of most …

ATAC‐seq: a method for assaying chromatin accessibility genome‐wide

JD Buenrostro, B Wu, HY Chang… - Current protocols in …, 2015 - Wiley Online Library
… A complete list of primers is available in Buenrostro et al. (2013). Care should be taken to
ensure that samples are barcoded appropriately for subsequent pooling and sequencing. …

[HTML][HTML] Chromatin potential identified by shared single-cell profiling of RNA and chromatin

…, T Law, C Lareau, YC Hsu, A Regev, JD Buenrostro - Cell, 2020 - cell.com
Cell differentiation and function are regulated across multiple layers of gene regulation,
including modulation of gene expression by changes in chromatin accessibility. However, …

Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position

JD Buenrostro, PG Giresi, LC Zaba, HY Chang… - Nature …, 2013 - nature.com
We describe an assay for transposase-accessible chromatin using sequencing (ATAC-seq),
based on direct in vitro transposition of sequencing adaptors into native chromatin, as a …

Single-cell chromatin accessibility reveals principles of regulatory variation

JD Buenrostro, B Wu, UM Litzenburger, D Ruff… - Nature, 2015 - nature.com
Cell-to-cell variation is a universal feature of life that affects a wide range of biological
phenomena, from developmental plasticity 1 , 2 to tumour heterogeneity 3 . Although recent …

Lineage-specific and single-cell chromatin accessibility charts human hematopoiesis and leukemia evolution

MR Corces, JD Buenrostro, B Wu, PG Greenside… - Nature …, 2016 - nature.com
We define the chromatin accessibility and transcriptional landscapes in 13 human primary
blood cell types that span the hematopoietic hierarchy. Exploiting the finding that the …

chromVAR: inferring transcription-factor-associated accessibility from single-cell epigenomic data

AN Schep, B Wu, JD Buenrostro, WJ Greenleaf - Nature methods, 2017 - nature.com
… Lareau for valuable suggestions for improvements on the package as well as members
of the Greenleaf and Buenrostro labs for useful discussions. … Jason D Buenrostro

Heritability enrichment of specifically expressed genes identifies disease-relevant tissues and cell types

…, Brainstorm Consortium, JRB Perry, JD Buenrostro… - Nature …, 2018 - nature.com
We introduce an approach to identify disease-relevant tissues and cell types by analyzing
gene expression data together with genome-wide association study (GWAS) summary …

[PDF][PDF] Integrated single-cell analysis maps the continuous regulatory landscape of human hematopoietic differentiation

JD Buenrostro, MR Corces, CA Lareau, B Wu… - Cell, 2018 - cell.com
… to explore chromatin accessibility differences in single-cell profiles (Buenrostro et al., 2015b).
Here, we … We thank members of Greenleaf, Chang, Majeti, and Buenrostro labs for valuable …