Phase transitions driven by intrinsically disordered protein regions (IDRs) have emerged as
a ubiquitous mechanism for assembling liquid-like RNA/protein (RNP) bodies and other …

HIV-1 Tat transactivation is vital for completion of the viral life cycle and has been implicated
in determining proviral latency. We present an extensive experimental/computational study …

The complex, interconnected architecture of cell-signaling networks makes it challenging to
disentangle how cells process extracellular information to make decisions. We have …

Liquid-liquid phase separation plays a key role in the assembly of diverse intracellular
structures. However, the biophysical principles by which phase separation can be precisely …

The optimization of engineered metabolic pathways requires careful control over the levels
and timing of metabolic enzyme expression 1 , 2 , 3 , 4 . Optogenetic tools are ideal for …

Altered glycolysis is a hallmark of diseases including diabetes and cancer. Despite intensive
study of the contributions of individual glycolytic enzymes, systems-level analyses of flux …

The ability to apply precise inputs to signaling species in live cells would be transformative
for interrogating and understanding complex cell-signaling systems. Here we report an '…

Optogenetic modules offer cell biologists unprecedented new ways to poke and prod cells.
The combination of these precision perturbative tools with observational tools, such as …

… Dine, E., Gil, AA, Uribe, G., Brangwynne, CP & Toettcher, JE Protein phase separation provides
… We thank all members of the Toettcher and Avalos laboratories for helpful comments. We …

… We thank members of the Toettcher and Shvartsman laboratories for comments and … system
we used in prior work in mammalian cells (Toettcher, et al., 2013; Toettcher, et al., 2011). We …