In microorganisms, noise in gene expression gives rise to cell-to-cell variability in protein
concentrations 1 , 2 , 3 , 4 , 5 , 6 , 7 . In mammalian cells, protein levels also vary 8 , 9 , 10 and …

Signal transduction pathways control cellular responses to stimuli, but it is unclear how
molecular information is processed as a network. We constructed a systems model of 7980 …

NADH is a key metabolic cofactor whose sensitive and specific detection in the cytosol of
live cells has been difficult. We constructed a fluorescent biosensor of the cytosolic NADH-NAD …

The EGF-stimulated ERK/MAPK pathway is a key conduit for cellular proliferation signals
and a therapeutic target in many cancers. Here, we characterize two central quantitative …

Apoptosis in response to TRAIL or TNF requires the activation of initiator caspases, which
then activate the effector caspases that dismantle cells and cause death. However, little is …

When exposed to tumor necrosis factor (TNF) or TNF-related apoptosis-inducing ligand (TRAIL),
a closely related death ligand and investigational therapeutic, cells enter a protracted …

The phosphoinositide 3-kinase (PI3K) pathway regulates multiple steps in glucose metabolism
and also cytoskeletal functions, such as cell movement and attachment. Here, we show …

10.7554/eLife.03342.001 Aerobic glycolysis or the Warburg Effect (WE) is characterized by
the increased metabolism of glucose to lactate. It remains unknown what quantitative …

Abstract Systems biology, particularly of mammalian cells, is data starved. However,
technologies are now in place to obtain rich data, in a form suitable for model construction and …

… Bissell and members of the Albeck and Bissell labs for critical reading of the manuscript and
helpful suggestions. We apologize to the authors of many excellent studies whose work we …