The diverse progenitors that give rise to the human neocortex have been difficult to
characterize because progenitors, particularly radial glia (RG), are rare and are defined by a …

Crossing the blood–brain barrier in primates is a major obstacle for gene delivery to the
brain. Adeno-associated viruses (AAVs) promise robust, non-invasive gene delivery from the …

… The neural stem cells, which Mich, Signer et al. term pre-GEPCOT cells (based on an acronym
of … Animal protocols were approved by the University of Michigan Committee on the Use …

During human brain development, multiple signaling pathways generate diverse cell types
with varied regional identities. Here, we integrate single-cell RNA sequencing and clonal …

Rapid cell type identification by new genomic single-cell analysis methods has not been met
with efficient experimental access to these cell types. To facilitate access to specific neural …

Viral genetic tools that target specific brain cell types could transform basic neuroscience
and targeted gene therapy. Here, we use comparative open chromatin analysis to identify …

Delivering genes to and across the brain vasculature efficiently and specifically across
species remains a critical challenge for addressing neurological diseases. We have evolved …

GABAergic interneurons are essential for neural circuit function, and their loss or dysfunction
is implicated in human neuropsychiatric disease. In vitro methods for interneuron …

The zebrafish has emerged as a versatile model organism for biomedical research, yet its
potential has been limited by a lack of conditional reverse-genetic tools. Here we report a …

Hedgehog (Hh) pathway activation and Gli-dependent transcription play critical roles in
embryonic patterning, tissue homeostasis, and tumorigenesis. By conducting a genome-scale …