… Author links open overlay panel Rime Kerfah 1 2 3 6 , Michael J Plevin 4 , Remy Sounier
5 , Pierre Gans 1 2 3 , Jerome Boisbouvier 1 2 3 … Boisbouvier …

Many microRNAs (miRNAs) are encoded by small gene families. In a third of all conserved
Arabidopsis miRNA families, members vary at two or more nucleotide positions. We have …

… (J) couplings between nuclei involved in Me/π interactions. Using an optimized isotope-labelling
strategy, these J … θ or ϕ on hπ J HMeCaro is similar to that seen for hπ J CMeCaro . …

Sometimes less is more: [13C1H3]methyl isotopomers can be biosynthetically incorporated
specifically into the pro-S methyl groups of leucine and valine residues in large protein …

A strategy for the introduction of ( 1 H, 13 C-methyl)-alanine into perdeuterated proteins is
described. Specific protonation of alanine methyl groups to a level of 95% can be achieved by …

MicroRNAs (miRNAs) derive from longer precursors with fold-back structures. While animal
miRNA precursors have homogenous structures, plant precursors comprise a collection of …

Atomic-resolution structure determination is crucial for understanding protein function. Cryo-EM
and NMR spectroscopy both provide structural information, but currently cryo-EM does …

… trans-hydrogen scalar coupling constants 2h J NN . The detection of a H-bond provides
direct information on base-pairing partners, and the 2h J NN coupling constant can be related to …

… Faustine Henot, Rime Kerfah, Ricarda Törner, Pavel Macek, Elodie Crublet, Pierre Gans,
Matthias Frech, Olivier Hamelin, Jerome Boisbouvier. Optimized precursor to simplify …

The function of proteins depends on their ability to sample a variety of states differing in
structure and free energy. Deciphering how the various thermally accessible conformations are …