Besides its essential and well established role as a component of the cytoskeleton, actin is
also present in the cell nucleus, where it has been linked to many processes that control gene …

We present a simple ratio method to infer protein composition within cellular structures using
proximity labeling approaches but compensating for the diffusion of free radicals. We used …

Actin has been linked to processes spanning the whole gene expression cascade, from
regulating specific transcription factors, such as myocardin-related transcription factor, to …

… J. Dopie was funded by a fellowship from the Helsinki Graduate Program in Biotechnology
and Molecular Biology. N. Perrimon is an investigator of the Howard Hughes Medical Institute. …

Nuclear actin plays an important role in many processes that regulate gene expression.
Cytoplasmic actin dynamics are tightly controlled by numerous actin-binding proteins, but …

… The initial recovery, when nuclear re-export is still negligible, can then be used as a measure
of nuclear import (Dopie et … The import assays was performed as described in Dopie et al. (…

Current models imply that the evolutionarily conserved, actin-binding Ezrin-Radixin-Moesin (ERM)
proteins perform their activities at the plasma membrane by anchoring membrane …

Actin controls numerous nuclear events including transcription factor activity, chromatin
remodeling and RNA polymerase activity. As a component of the cytoskeleton in the cytoplasm, …

… The findings of Dopie and colleagues emphasise how important it is to assess whether the
interactions between actin and nuclear targets are dynamic or stable. Short-term variations in …

Nuclear actin has been implicated in a variety of DNA-related processes including
chromatin remodeling, transcription, replication, and DNA repair. However, the mechanistic …