Cardiac resuscitation after cardiac arrest or ventricular fibrillation has been limited by the
need for open thoracotomy and direct cardiac massage. As a result of exhaustive animal …

Contractility of airway smooth muscle requires elevation of intracellular calcium
concentration. Under resting conditions, airway smooth muscle cells maintain a relatively low …

Following the discovery of BRD4 as a non-oncogene addiction target in acute myeloid
leukaemia (AML) 1 , 2 , bromodomain and extra terminal protein (BET) inhibitors are being …

Defining direct targets of transcription factors and regulatory pathways is key to
understanding their roles in physiology and disease. We combined SLAM-seq [thiol(SH)–linked …

Cellular differentiation involves profound remodelling of chromatic landscapes, yet the
mechanisms by which somatic cell identity is subsequently maintained remain incompletely …

A new method of producing artificial circulation by external sternal compression without
thoracotomy was evaluated in 138 episodes of cardiac arrest in 118 patients. Seventy-six arrests …

Phosphatidylinositol-5-phosphate (PtdIns5P)-4-kinases (PIP4Ks) are stress-regulated lipid
kinases that phosphorylate PtdIns5P to generate PtdIns(4,5)P 2 . There are three isoforms of …

… (I) Live cells stably expressing the nuclear import substrate IBB-EGFP were imaged 96 hr
after siRNA transfection and (J) analyzed for the ratio of nuclear over cytoplasmic concentration …

Protein expression and turnover are controlled through a complex interplay of transcriptional,
post-transcriptional and post-translational mechanisms to enable spatial and temporal …

CRISPR–Cas9 screens have emerged as a transformative approach to systematically probe
gene functions. The quality and success of these screens depends on the frequencies of …