The tri-snRNP 27K protein is a component of the human U4/U6-U5 tri-snRNP and contains
an N-terminal phosphorylated RS domain. In a forward genetic screen in C. elegans, we …

The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to augment …

In Caenorhabditis elegans, germline apoptosis is promoted by egl-1 and ced-13 in response
to meiotic checkpoint activation. We report that the requirement for these two factors …

Pre-mRNA splicing must occur with extremely high fidelity. Spliceosomes assemble onto pre-mRNA
guided by specific sequences (5′ splice site, 3′ splice site, and branchpoint). …

In sexually reproducing metazoans, spermatogenesis is the process by which uncommitted
germ cells give rise to haploid sperm. Work in model systems has revealed mechanisms …

Auxin-inducible degradation is a powerful tool for the targeted degradation of proteins with
spatiotemporal control. One limitation of the auxin-inducible degradation system is that not all …

Spermatogenesis is the process through which mature male gametes are formed and is
necessary for the transmission of genetic information. While much work has established how …

Nematode molting is a remarkable process where animals must repeatedly build a new apical
extracellular matrix (aECM) beneath a previously built aECM that is subsequently shed. …

Adjacent alternative 3′ splice sites, those separated by ≤18 nucleotides, provide a unique
problem in the study of alternative splicing regulation; there is overlap of the cis-elements …

Lysosomes are an important organelle required for the degradation of a range of cellular
components. Lysosome function is critical for development and homeostasis as dysfunction can …