Giardia lamblia is a ubiquitous intestinal pathogen of mammals. Evolutionary studies have
also defined it as a member of one of the earliest diverging eukaryotic lineages that we are …

Giardia lamblia is an extremely primitive or early-diverging eukaryote that has been considered
to have no typical ER or Golgi apparatus, although it is a complex and highly developed …

Important Ca 2+ signals in the cytosol and organelles are often extremely localized and hard
to measure. To overcome this problem we have constructed new fluorescent indicators for …

We have recently characterized three yeast gene products (Vps35p, Vps29p, and Vps30p)
as candidate components of the sorting machinery required for the endosome-to-Golgi …

Many cellular events depend on a tightly compartmentalized distribution of H + ions across
membrane-bound organelles. However, measurements of organelle pH in living cells have …

Mitochondria are highly mobile and dynamic organelles that continually fuse and divide.
These processes allow mitochondria to exchange contents, including mitochondrial DNA (…

Vesicle fusion involves vesicle tethering, docking, and membrane merger. We show that
mitofusin, an integral mitochondrial membrane protein, is required on adjacent mitochondria to …

Mutations in the mitochondrial fusion gene Mfn2 cause the human neurodegenerative disease
Charcot-Marie-Tooth type 2A. However, the cellular basis underlying this relationship is …

The dynamin-related GTPase Dnm1 controls mitochondrial morphology in yeast. Here we
show that dnm1 mutations convert the mitochondrial compartment into a planar ‘net’of …

Non-amyloid, ubiquitinated cytoplasmic inclusions containing TDP-43 and its C-terminal
fragments are pathological hallmarks of amyotrophic lateral sclerosis (ALS), a fatal motor …