[HTML][HTML] Engineering and Two-Stage Evolution of a Lignocellulosic Hydrolysate-Tolerant Saccharomyces cerevisiae Strain for Anaerobic Fermentation of Xylose from …

…, C Li, D Tanjore, IM Ong, H Li, EL Pohlmann, J Serate… - PloS one, 2014 - journals.plos.org
The inability of the yeast Saccharomyces cerevisiae to ferment xylose effectively under
anaerobic conditions is a major barrier to economical production of lignocellulosic biofuels. …

Assessing the viability of recovery of hydroxycinnamic acids from lignocellulosic biorefinery alkaline pretreatment waste streams

SD Karlen, P Fasahati, M Mazaheri, J Serate… - …, 2020 - Wiley Online Library
The hydroxycinnamic acids p‐coumaric acid (pCA) and ferulic acid (FA) add diversity to the
portfolio of products produced by using grass‐fed lignocellulosic biorefineries. The level of …

[HTML][HTML] Controlling microbial contamination during hydrolysis of AFEX-pretreated corn stover and switchgrass: effects on hydrolysate composition, microbial response …

J Serate, D Xie, E Pohlmann, C Donald… - Biotechnology for …, 2015 - Springer
Background Microbial conversion of lignocellulosic feedstocks into biofuels remains an
attractive means to produce sustainable energy. It is essential to produce lignocellulosic …

[HTML][HTML] Inhibition of microbial biofuel production in drought-stressed switchgrass hydrolysate

…, Q Dickinson, D Xie, SA Smith, J Serate… - Biotechnology for …, 2016 - Springer
Background Interannual variability in precipitation, particularly drought, can affect
lignocellulosic crop biomass yields and composition, and is expected to increase biofuel yield …

Diverse lignocellulosic feedstocks can achieve high field‐scale ethanol yields while providing flexibility for the biorefinery and landscape‐level environmental benefits

Y Zhang, LG Oates, J Serate, D Xie… - GCB …, 2018 - Wiley Online Library
Increasing the diversity of lignocellulosic feedstocks accepted by a regional biorefinery has
the potential to improve the environmental footprint of the facility; harvest, storage, and …

Mutagenesis and functional characterization of the four domains of GlnD, a bifunctional nitrogen sensor protein

Y Zhang, EL Pohlmann, J Serate… - Journal of …, 2010 - Am Soc Microbiol
GlnD is a bifunctional uridylyltransferase/uridylyl-removing enzyme (UTase/UR) and is believed
to be the primary sensor of nitrogen status in the cell by sensing the level of glutamine in …

[HTML][HTML] Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway

…, JF Wolters, M Place, JJ Dietrich, D Xie, J Serate… - Synthetic and Systems …, 2022 - Elsevier
Metabolic engineering strategies have been successfully implemented to improve the
production of isobutanol, a next-generation biofuel, in Saccharomyces cerevisiae. Here, we …

[HTML][HTML] Multiomic fermentation using chemically defined synthetic hydrolyzates revealed multiple effects of lignocellulose-derived inhibitors on cell physiology and …

Y Zhang, JM Vera, D Xie, J Serate… - Frontiers in …, 2019 - frontiersin.org
Utilization of both C5 and C6 sugars to produce biofuels and bioproducts is a key goal for the
development of integrated lignocellulosic biorefineries. Previously we found that although …

[HTML][HTML] Characterization of variants altered at the N-terminal proline, a novel heme-axial ligand in CooA, the CO-sensing transcriptional activator

…, CR Staples, WN Lanzilotta, TJ Poulos, J Serate… - Journal of Biological …, 2000 - ASBMB
CooA, the carbon monoxide-sensing transcription factor from Rhodospirillum rubrum, binds
CO through a heme moiety resulting in conformational changes that promote DNA binding. …

[HTML][HTML] Production of hydrolysates from unmilled AFEX-pretreated switchgrass and comparative fermentation with Zymomonas mobilis

Y Zhang, J Serate, D Xie, S Gajbhiye, P Kulzer… - Bioresource Technology …, 2020 - Elsevier
The biological route for producing biofuel from lignocellulosic biomass involves many steps,
including biomass harvesting and processing, pretreatment, enzymatic hydrolysis, microbial …