Both the generation and the analysis of proteomics data are now widespread, and high-throughput
approaches are commonplace. Protocols continue to increase in complexity as …

A combination of high‐resolution two‐dimensional (2‐D) polyacrylamide gel electrophoresis,
highly sensitive biological mass spectrometry, and the rapidly growing protein and DNA …

The actin-binding proteins of the actin-depolymerisation factor (ADF)/cofilin family were first
described more than three decades ago, but research on these proteins still occupies a front …

Limited action of papain on the native forms of two cellobiohydrolases (CBH) from Trichoderma
reesei (CBH I, 65 kDa, and CBH II, 58 kDa) leads to the isolation of the respective core …

The generation of invasiveness in transformed cells represents an essential step of tumor
progression. We have previously shown that MDCK epithelial cells, which are deprived of …

… actins, changes of charged amino acid residues are restricted to this peptide (Vandekerckhove …
This approach has been reported in detail elsewhere (Vandekerckhove …

We identified four polypeptides of 47, 44, 40, and 35 kD that bind to profilin-Sepharose and
elute with high salt. When purified by conventional chromatography using an antibody to the …

Current non-gel techniques for analyzing proteomes rely heavily on mass spectrometric
analysis of enzymatically digested protein mixtures. Prior to analysis, a highly complex peptide …

Scatter factor (SF), a secretory protein of fibroblasts, dissociates and increases the motility of
epithelial cells and may be involved in cell migration processes during embryogenesis and …

N α -terminal acetylation is one of the most common protein modifications in eukaryotes. The
COmbined FRActional DIagonal Chromatography (COFRADIC) proteomics technology that …