User profiles for Jack Bravo
 | Jack BravoUniversity of Texas at Austin Verified email at ista.ac.at Cited by 613 |
[HTML][HTML] Structural basis for mismatch surveillance by CRISPR–Cas9
CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA cleavage
1 , 2 , 3 – 4 , and the underlying mechanisms by which Cas9 recognizes mismatches are …
1 , 2 , 3 – 4 , and the underlying mechanisms by which Cas9 recognizes mismatches are …
[PDF][PDF] Remdesivir is a delayed translocation inhibitor of SARS-CoV-2 replication
Remdesivir is a nucleoside analog approved by the US FDA for treatment of COVID-19.
Here, we present a 3.9-Å-resolution cryo-EM reconstruction of a remdesivir-stalled RNA-…
Here, we present a 3.9-Å-resolution cryo-EM reconstruction of a remdesivir-stalled RNA-…
[HTML][HTML] Structural basis for assembly of non-canonical small subunits into type IC Cascade
Bacteria and archaea employ CRISPR (clustered, regularly, interspaced, short palindromic
repeats)-Cas (CRISPR-associated) systems as a type of adaptive immunity to target and …
repeats)-Cas (CRISPR-associated) systems as a type of adaptive immunity to target and …
Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses
RNA viruses induce the formation of subcellular organelles that provide microenvironments
conducive to their replication. Here we show that replication factories of rotaviruses …
conducive to their replication. Here we show that replication factories of rotaviruses …
[HTML][HTML] SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation
Characteristic properties of type III CRISPR-Cas systems include recognition of target RNA
and the subsequent induction of a multifaceted immune response. This involves sequence-…
and the subsequent induction of a multifaceted immune response. This involves sequence-…
[HTML][HTML] RNA targeting unleashes indiscriminate nuclease activity of CRISPR–Cas12a2
Cas12a2 is a CRISPR-associated nuclease that performs RNA-guided, sequence-nonspecific
degradation of single-stranded RNA, single-stranded DNA and double-stranded DNA …
degradation of single-stranded RNA, single-stranded DNA and double-stranded DNA …
[HTML][HTML] Structural rearrangements allow nucleic acid discrimination by type ID Cascade
CRISPR-Cas systems are adaptive immune systems that protect prokaryotes from foreign
nucleic acids, such as bacteriophages. Two of the most prevalent CRISPR-Cas systems …
nucleic acids, such as bacteriophages. Two of the most prevalent CRISPR-Cas systems …
[HTML][HTML] A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome
RNA modifications are widespread in biology and abundant in ribosomal RNA. However,
the importance of these modifications is not well understood. We show that methylation of a …
the importance of these modifications is not well understood. We show that methylation of a …
[HTML][HTML] Structural basis for broad anti-phage immunity by DISARM
JPK Bravo, C Aparicio-Maldonado, FL Nobrega… - Nature …, 2022 - nature.com
In the evolutionary arms race against phage, bacteria have assembled a diverse arsenal of
antiviral immune strategies. While the recently discovered DISARM (Defense Island System …
antiviral immune strategies. While the recently discovered DISARM (Defense Island System …
Type III-B CRISPR-Cas cascade of proteolytic cleavages
The generation of cyclic oligoadenylates and subsequent allosteric activation of proteins that
carry sensory domains is a distinctive feature of type III CRISPR-Cas systems. In this work, …
carry sensory domains is a distinctive feature of type III CRISPR-Cas systems. In this work, …